Uma Maheshwar

About Uma Maheshwar

PROJECTS INVOLVED:1.Analytical test method development,qualification and validation for Viral vaccines (Rota virus vaccine and Hep-A vaccine) Gene Therapy products, petides and for nano particle based formulations2. Development, Qualification and validation of Cell based assays.3. Development, Qualification of NAb assays.4. Development and Qualification HCP and Benzonase ELISA assays.5. Development of Analytical Ultra centrifugation methods6. Studying of Apoptosis (Cas-9) in Human epithelial cell lines upon Japanese encephalitis Virus Infection.7.Modulation of Non classical MHC molecules expression in epithelial cell lines upon Japanese encephalitis Virus Infection.8.Expression & Purification of Recombinant Human serum albumin.9. Optimization of expression and purification of rEPO in Pichia pastoris host using Shake flask experiments.Specialties: • Handling of Human,Mouse cell lines • Infection of Cell lines with Virus.• Analytical test method development and validation for Viral vaccines• Handling on experience to Concentrate Fermentation broth by Sartorius Tangential Ultra filtration (TFF).• Chromatographic Columns like Ion exchange, Gel filtration,HIC and RPC • SDS-PAGE• Western blotting• 2-D gel electrophoresis.• Cultivation and maintenance of E. coli and Pichia strains.

Experience

1. General Manager

   ALS

   Sep 2024 — Present · Hyderabad, IN

Education

• Silver jubilee degree college, (APRDC),kurnool

  B.Sc., Microbiology

• Kakatiya University, Warangal

  Master of Science, Microbiology

• Indian Institute of Management Bangalore

  Micro Masters in Business Management through edX

  2020 — 2021