Wenjing Chen

WORK HISTORY

EDUCATION

SKILLS

ABOUT WENJING CHEN

Scientist with over 12 years of research experience, having expertise in protein purification, expression and characterization, molecular biology and enzymology. A committed and self-driven researcher • Solid background and expertise in recombinant Protein/Antibody expression/purification and characterization (protein electrophoresis, affinity chromatography, SEC/IEC/HIC, AKTA, HPLC, IEF, protein crystallization, western blots, ELISA) in both mammalian and bacterial system. • Protein functional assay development including spectrophotometric assay (96-well and 384-well format), radioactive assay, high-throughput screening assay, etc.• Biochemical and analytical characterization including protein electrophoresis, LC-MS, NMR, affinity chromatography, HPLC/SEC/IEC, NMR, TLC, bioTLC, and protein crystallization, IC50, MIC determination, etc.• Recombinant protein expression/purification Centrifugation, homogenization, denaturation, refolding, solving insoluble recombinant potein problem), protein chemistry and protein engineering, saccharide chemistry. • Extended experience in molecular cloning techniques including genomic DNA isolation, PCR, DNA extraction, vector construction, mutagenesis, electrophoresis, knockouts, etc.• Extensive experience in pathogenic and non-pathogenic microbes. Isolation, culture, storage and archiving of various microorganisms (such as E. coli, Bacillus anthracis, Bacillus subtilis, Streptomyces lasaliensis, Kutzneria spp, Streptomyces rishiriensis, Mycobacterium smegmatis, Mycobacterium tuberculosis, Serratia marcescens, etc).• Attention to detail; Strong ability to work independently as well as collaboratively in an interdisciplinary environment; capable of project management; willing to learn and progress; proficient in GraphPad Prism, Sigmaplot, Kaleidagraph