Prakruthi Vadakattu
Research Technologist @Mayo Clinic
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WORK HISTORY
Research Technologist @Mayo Clinic
Jacksonville, FL, US
EDUCATION
Manipal Academy of Higher Education
Bachelor of Technology - BTech, Biomedical Engineering
Brown University
Master of Science , Bioengineering and Biomedical Engineering
ABOUT PRAKRUTHI VADAKATTU
AboutI am Prakruthi Vadakattu, a Biomedical Engineer with a Master’s degree from Brown University, currently working at the Mayo Clinic Neuroscience Department.My work focuses on understanding neurodegenerative mechanisms through immuno-pluripotent stem cell (iPSC) models, extracellular vesicle (EV) biology, and molecular neuroscience techniques.I bring experience across cell culture, molecular assays, immunohistochemistry (IHC), confocal imaging, and data-driven analysis, combining scientific precision with translational research insight.Key Highlights-iPSC Differentiation & Characterization: Developed and optimized reproducible differentiation protocols into microglia, astrocytes, and neurons for disease modeling and functional analysis-Extracellular Vesicle Isolation & Profiling: Led end-to-end EV isolation and characterization workflows using NanoFCM and NTA to analyze vesicle concentration, size, and cargo distribution-Advanced Imaging & Quantitative Analysis: Executed immunohistochemistry and confocal microscopy studies to investigate neuroinflammatory signaling and protein localization-Quality Control & Laboratory Management: Maintained contamination-free stem cell culture environments by leading mycoplasma testing and standardizing aseptic workflows across the lab-Cross-Functional Collaboration: Partnered with multidisciplinary research teams to integrate molecular, imaging, and cellular data into cohesive translational neuroscience projects.I am passionate about bridging stem cell biology and translational neuroscience to drive innovations in disease modeling, biomarker discovery, and therapeutic development.My long-term goal is to contribute to the advancement of neuroscience-driven biotech research through rigorous experimentation, data interpretation, and cross-domain collaboration.Open to connecting with professionals and organizations in the fields of biotechnology, neurobiology, and cellular therapeutics.
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