Patricia Legler

Pi (Research Biologist 0401) @U.S. Naval Research Laboratory

Washington, DC, US

o Principle Investigator (PI) & Co-PI & Co-Io Wrote grants (25%), performed lab work (75%), trained students, oversaw budget, tasks, milestones, SOW, reportso Undergraduate student training – trained >20 students o Characterized viral proteases, host substrates, bacterial enzymes, human esterases and other enzymes using in vitro assayso Western blotting, cell-based assays (protease), single domain antibody characterizationo Protein Expression & Purification Large Scale (500 mg- 1 gram) – Bacterial (E.coli) & Mammalian (Expi293 cells)o Viral genome analysis – Developed the first sequence-to-symptom software for viruses. Extracted symptoms directly out of the viral genome sequence using clusteringo Machine Learning (ML) models for viral genomic analysiso In silico proteomic studies (Bioinformatics) for viral protease substrates in humans (Python)o Measured kinetic parameters for viral proteases (Km, Vmax, kcat)o Cell culture for mammalian protein expression, viral proteases o Developed and characterized novel REE binding peptides using in vitro HTS assays and FRET substrates for Kd, screened phage display librarieso Machine learning model analysis for metal binding peptides using Wekao Technology transfer of novel engineered biomaterials to a mining companyo Developed HTS assays for viral protease inhibitors and screened compounds from NCATS/NIH in silico screens. Measured IC50s, Kd, and Kio Plate Reader assays (HTS)- fluorescence, UV-vis, FRETo Kinetic analysis of small molecule inhibitors of human esterases (AChE, Cathepsin A, SFGH, hCE1), gamma glutamyltransferases (GGT), and proteases (SARS-CoV-2, ZIKV, VEEV, botulinum neurotoxin zinc metalloproteinase)o Pegylationo Kinetic analysis of mouse sera for PK studieso Circular Dichorism (Tm)o X-ray Crystallographyo Sought external funding from DTRA, CDMRP, JSTO-RISE, and OUSDo CBRN Subject Matter Expert, Chemical Surety, Select Agentso Published 47 papers in peer-reviewed journals

Washington University in St. Louis

B.A.

The Johns Hopkins University School of Medicine

PhD