Nalaka Rannulu
Senior Principal Scientist @Johnson & Johnson Innovative Medicine
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WORK HISTORY
Senior Principal Scientist @Johnson & Johnson Innovative Medicine
Malvern, PA, US
EDUCATION
University of Kelaniya, Sri Lanka
B.Sc. Chemistry special (Hons)
Wayne State University
Ph.D., Chemistry (Analytical)
University of Peradeniya
M.Phil., Analytical Chemistry
SKILLS
ABOUT NALAKA RANNULU
In-depth knowledge and hands-on experience in mass spectrometry for identification, characterization, and quantification of small molecules and larger proteins. Experienced in development of novel electrospray methods for proteins and small molecules. Expertise in working with different fragmentation methods: CID, SORI CID, ECD, and ETD. Experienced in development of automated, data-independent ESI MS/MS in conjunction with nanocapillary LC and database searching for identification of proteins in biological samples.Specialties: Mass spectrometry- Operation, maintenance, and troubleshooting of multiple mass spectrometry platforms, mass spectrometry method development and validation, protein/peptide identification, characterization, and quantifications, sample extraction, proteolytic digestion, post-digest sample clean-up and preparation for mass spectrometrySeparation methods- HPLC-based chromatographic techniques (size exclusion, ion exchange, reversed phase, HILIC glycan), CE-SDS, cIEF, SDS-PAGE, GC, GC-MSComputer skills - Microsoft office, Electronic lab notebook, Endnote, Sigma plots, Scifinder, ChemDrawProteomics/bioinformatics software- • Empower • Masshunter •Thermo Xcalibur •Mass Analyzer • 32karot • Applied Biosystems Analyst •Waters MassLynx •Bruker apex control •Waters ProteinLynx Global Server(PLGS) •Waters Expression Analysis, MASCOT
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