Manish Moghe

Research Associate Iii (2017-present) Phd Candidate (2019-2024) @Lombardi Comprehensive Cancer Center

Washington, DC, US

Assessed in vivo molecular imbalance caused by toxin exposure to facilitate target identification.• Established a surrogate invitro model with molecular assays as readouts to screen the efficacy and toxicity of candidate siRNA and small molecule-based payloads invitro.• Established the animal model and performed the in vivo validation and toxicity evaluation for candidates.• Evaluated key neuroinflammatory consequences caused by the exposure like cytokine surge, gliosis, and neuronal loss in animal model using molecular and microscopy-based assays.• Examined the ability of the lead candidates to alleviate the consequences of disorder.• Processed data, maintained lab reports, and presented the progress reports to the principal investigators and in department meetings.Skill list:Molecular Biology: RT-PCR NanoString ELISA Nucleic Acid Extraction Protein Extraction Cell Biology: Mammalian cell culture Transfection In Vivo (Mouse) Intra-nasal, intra-peritoneal, subcutaneous administration Rodent radiation therapy Tissue harvest Brain Dissection Blood collection Mice perfusion.Imaging: Fluorescence microscopy Tissue histology analysis

Padmashree Dr D. Y. Patil Vidyapeeth

Bachelor's degree

Georgetown University

Doctor of Philosophy - PhD

Mahatma School of Accademics and Sports

SSC

Georgetown University

Master’s Degree

Mahatma Education society

High School