Harikrishna Thulaseedharan
Cirm Intern @UC San Diego Health
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WORK HISTORY
Cirm Intern @UC San Diego Health
San Diego, CA, US
Developing ASO and CRISPR/Cas9-based therapeutic strategies for correcting genetic mutations in pediatric brain disease models (focus: PACS1-associated disorders).🧬 Culturing and maintaining induced pluripotent stem cells (iPSCs) and assisting in neuronal differentiation to model human neurodevelopment. Supporting ASO treatment experiments through design, application, and RNA expression analysis.🧪 Assisting in CRISPR genome editing workflows, including reagent preparation, transfection, and validation of edits. Utilizing microscopy and imaging techniques to monitor neuronal morphology and disease phenotypes. Contributing to differential gene expression (DGE) analysis to identify biomarkers of therapeutic outcomes.🤝 Collaborating across molecular biology, stem cell culture, and imaging experiments to advance personalized gene-targeted therapies for pediatric neurological disorders.
ABOUT HARIKRISHNA THULASEEDHARAN
I am a PharmD-trained biotechnology graduate researcher with current Master’s training in…
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