Younghwan Kim
Senior Engineer Metabolic Engineer @CleanJoule
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WORK HISTORY
Senior Engineer Metabolic Engineer @CleanJoule
Laboratory setup and management • Sustainable aviation fuel (SAF) bioprocessing system development• Design of experiment (DOE), complex problem-solving, and learning new methods• Gene knockout (CRISPR-Cas9, KEIO), knockdown, protein overexpression, etc.• Developing multiple microbes (fungus, bacteria) to overexpress SAF molecules by modifying metabolic pathways
EDUCATION
University of Utah John and Marcia Price College of Engineering
Doctor of Philosophy - PhD, Materials Science and Engineering - Metallurgical Engineering
Sungkyunkwan University
Bachelor of Science - BS, Genetic Engineering
Sungkyunkwan University
Master of Science - MS, Genetic Engineering
University of Utah John and Marcia Price College of Engineering
Master of Engineering - MEng, Metallurgical Engineering
SKILLS
ABOUT YOUNGHWAN KIM
I am an interdisciplinary scientist and engineer with over 20 years of experience in biotechnology and nanomaterial engineering for medical devices. Career Highlights- Genetic Engineering Expertise: With ~15 years in the biotech industry and academia, I have honed my skills in gene manipulation for mammalian and bacterial strain development, from plasmid vector design to antibody titer testing- Materials Engineering Proficiency: My experience includes nanomaterial fabrication and biosensor system development, working on projects involving titanium dioxide nanotubes, and creating a peptide nucleic acid (PNA)-TiO2 biosensor for tuberculosis- Bioprocess Optimization for Renewable Bioenergy: Proficient in designing and optimizing upstream bioprocesses for scalable production, using techniques such as vector design, cloning, and strain development to enhance biofuel precursor yields- Protein Purification: Hands-on expertise in cloning, protein overexpression, purification, and activity testing for various biotechnological applications- Technical Writing and Regulatory Knowledge: Proficient in writing technical reports, publications, and patent filings, ensuring meticulous documentation and compliance with industry standards- Project Management: Effectively managed multiple projects, overseeing proposal writing, budget estimation, and timeline management to achieve project objectives within designated constraints. Lean Sigma Six Green Belt Certificate. Areas of Specialization- Analytical Skills: Experienced in using SEM, EDX, fluorescent and confocal microscopy, sequencing analysis, FPLC, qRT-PCR, GC, HPLC, Raman spectroscopy, UV-Vis, siRNA, shRNA, IHC, ELISA, potentiostat, and various molecular biology techniques- Software tools: MS Office, Gantt chart, R, SnapGene, EndNote, LaTeX, etc- Biomedical Devices: Breath analyzer biosensor platforms and designing innovative titanium electrode systems- Nanomaterials Fabrication: Solvent-based anodization methods, tube furnace annealing, calcination, sputtering, metal casting, etc- Neuroscience & Neurodegenerative Diseases: Research on prion diseases and brain degenerative diseases using neuronal cell lines and developing transient cell lines- Vaccines: Developing recombinant vaccines and GMO plants for edible vaccine delivery- Antibodies: Generating antibodies from mice and rabbits for vaccine development- Model Animal Management: Managing transgenic mice, including mating, genotyping, embryo analysis, and zonde i••••••••@hotmail.com
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