Yash Kulkarni

Research Technologist- Neural and Behavioral Sciences @Penn State College of Medicine

Hershey, PA, US

Spearhead spatial transcriptomic profiling of 100 knockout mouse brains using Vizgen MERSCOPE Ultra, driving critical insights for a high-impact, multi-institutional consortium initiative- Execute ultra-precise single-cell isolation using Cellenone X1, enabling downstream single-cell transcriptomic and proteomic analyses with high fidelity- Design, optimize, and perform complex molecular biology assays in cancer models, including qPCR, western blotting, immunoprecipitation, chromatin extraction, colony formation, and cell proliferation studies- Elucidate the functional relationship between DNA polymerases and chromatin remodelers in both neural and cancer cell systems, integrating mechanistic biology with disease relevance- Drive gene knockdown strategies via CRISPR-Cas9, siRNA, and viral shRNA approaches, establishing robust functional genomics platforms across diverse cell models- Mentor and train PhD students and junior researchers in advanced molecular techniques and experimental design, fostering technical excellence and collaborative learning- Oversee and optimize laboratory operations, including inventory control, equipment maintenance, and workflow management to ensure high lab efficiency and compliance- Perform rigorous bioinformatic and statistical analyses of large-scale cancer datasets using R, TCGA, and GEO, translating complex omics data into actionable biological insights.

Penn State College of Medicine

Master's degree, Neuroscience

DY Patil University

Bachelor of Technology - BTech, Biotechnology