Yangjie Wei

About Yangjie Wei

Specialties: • Formulation development: High-throughput excipient screening, Accelerated stability study, Design of experiment, Physical degradation studies (thermal and shaking stress), Formulation experience on small molecules, proteins, virus, and mRNA• Protein Expression and Purification: Protein expression in E.coli system, Protein purification using ÄKTA FPLC system, Ultrafiltration/Diafiltration (UF/DF)• Protein biophysical and analytical characterization: SDS-PAGE, Circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, Fluorescence spectroscopy, Differential scanning Flourimetry (DSF), UV/Vis spectroscopy, Differential scanning calorimetry (DSC), Dynamic light scattering (DLS), Zeta potential, Multiangle light scattering (MALS), Micro-flow imaging (MFI), Bio-Layer Interferometry (BLI), Viscometer, Densitometer, ELISA, Western blot• Protein conjugation: Formaldehyde crosslinking, Gold nanoparticle/protein conjugation• Chromatographic techniques: Hydrophobic interaction chromatography (HIC), Size-exclusion chromatography (SEC), Ion exchange chromatography (IEX), and Immobilized metal affinity chromatography (IMAC), Reverse phase chromatography (RP-HPLC), Validation of analytical procedures according to ICH Guidelines• Small molecule characterization: lyophilization, In-vitro drug release, 1H NMR• Software and data analysis: Pymol, JMP, Endnote, Empower, Electronic lab notebook, Origin, Python

Experience

1. Principal Scientist

   Amgen

   Jul 2023 — Present

Education

• China Pharmaceutical University

  Bachelor of Science (BS), Pharmaceutics

• The University of Toledo

  Master of Science (M.S.), Industrial Pharmacy

• The University of Kansas

  Doctor of Philosophy (PhD), Pharmaceutical Chemistry

  2014 — 2018

Skills

• Differential Scanning Calorimetry
• Fluorescence Spectroscopy
• Intrinsic Fluorescence
• Dls
• Cell Culture
• Cell Biology
• Ft-Ir
• Formulation
• Raman Spectroscopy
• Ftir
• Immunohistochemistry
• Polymerase Chain Reaction (Pcr)
• Circular Dichroism
• Microscopy
• Flow Cytometry
• Light Scattering
• Elisa
• Western Blotting
• High-Performance Liquid Chromatography (Hplc)
• Bio-Layer Interferometry
• Confocal Microscopy
• Protein Expression
• Protein Chemistry
• Molecular Cloning
• Hplc
• Molecular Biology
• Fluorescence Microscopy
• Pcr
• Biochemistry
• Protein Purification
• Viscometry