Yanbo Zhang

About Yanbo Zhang

Team and Biology Lead specializing in non-clinical research, from early discovery throughout IND approval, for drug development targeting immunology and inflammation (I&I) diseases.Immunologist by training in adaptive immunity (T cell, Treg) and innate immunity (macrophage), at the levels of molecular, cellular, and systems immunology; working on both in vitro/ex vivo human and murine immune cell-based assays and in vivo disease models.* Specialties: immune tolerance, Regulatory T cell biology, myeloid cell biology, immuno-oncology, autoimmune diseases.Highlights:* CRISPR/Cas9 KO for genetic screening (pool and individual), target discovery and validation in primary immune cells in vivo and in vitro/ex vivo, and mechanism of action studies;* Characterization of various immune cell subsets in vivo and in vitro, using multi-color flow cytometry, immunophenotyping, RNAseq, and analysis (differential expression, signature, enrichment, etc.).* Various mouse disease models: tumor, autoimmune disease, infectious disease, colitis, bone marrow chimeras;* Primary immune cell isolation, sorting, and manipulation;* Primary immune cell culture (T cells, Regulatory T cells /Tregs, macrophages, DCs), various immune cell-based functional assays, and cell-based assay development.* Gene overexpression and genetic knock-out in primary immune cells and various cell lines;* Hematopoietic stem cell (HSC) isolation, genetic manipulation, and transplantation;* Working with PBMC and patient blood samples;* End-to-end lentivirus library and NGS sequencing library construction.* Former trainee of Dr. Christophe Benoist and Dr. Diane Mathis at the Department of Immunology, Harvard Medical School.

Experience

1. Associate Director, Biology

   Nimbus Therapeutics

   Oct 2024 — Present · Boston, MA, US

Education

• Chinese Academy of Sciences

  Doctor of Philosophy (Ph.D.), Cell Biology (focused on Immunology)

  2008 — 2014

• Henan University

  Bachelor's degree, Biotechnology

Skills

• Pcr
• Bone Marrow Transplantation
• Flow Cytometry
• Elisa
• Chromatin Immunoprecipitation
• Single Molecule Biology
• Atomic Force Microscope
• R
• Protein Expression
• Microscopy
• Single Molecule Force Spectroscopy
• Genetics
• Cas9
• Mouse Models
• Immunoprecipitation
• Cell Sorting
• Biochemistry
• Molecular Biology
• Fluorescence Microscopy
• Genetic Engineering
• Gene Expression Profiling
• Microbiology
• Genetic Editing
• Cell Biology
• Molecular Cloning
• Animal Models
• Immunology
• Single Cell Force Spectroscopy
• Pcr Primer Design
• Embryonic Stem Cells
• Confocal Microscopy
• Tumor Immunology
• Immune Disorders
• Immunohistochemistry
• Qpcr
• Immunofluorescence
• Immune System
• Biotechnology
• Protein Purification
• Crispr