Vladimir Kuznetsov

About Vladimir Kuznetsov

High-Performing, Results-Driven Research Scientist with 12+ years of successful experience in developing monoclonal antibodies, fusion proteins, vaccines, and gene therapy products• Experienced in broad molecular biology;• Extensive cell culture experience: primary and lineage cells, maintenance, cell bank management, transient and stable transfection, transduction, clone selection, and protein expression;• Hands-on experience in monoclonal, polyclonal, single-chain variable fragment (scFv), recombinant, chimeric and humanized antibody development, production, purification, engineering and characterization;• Accomplished in developing cell culture medium, feeding strategies, and process parameters optimization;• Practiced in upstream and downstream process development for biologics, utilizing design of experiments (DOE) and quality by design (QbD) approach;• Expert in low and high-pressure chromatography: FPLC, HPLC, LC-MS, IEX, SEC, HIC, affinity and tag-assisted; protein analysis: electrophoresis, IEF, DLS, enzyme activity assays and kinetics;• Skilled in protein crystallography, including soluble and membrane proteins, macromolecular complexes, and protein-ligand interactions. Proficient in X-ray diffraction data collection (home and synchrotron sources) and structure solving using molecular replacement and SAD/MAD phasing. • Proven success in leading multi-disciplinary and cross-functional teams toward actionable/measurable goals utilizing technology transfer and scale-up.

Experience

1. Associate Director, Vector Core Process Development

   Life Edit Therapeutics

   Mar 2024 — Present · Durham, NC, US

Education

• University of California, Santa Cruz

  Bachelor of Science - BS, Chemistry

  1999

• Texas A&M University

  Doctorate of Philosophy, Chemistry

  2011

Skills

• Cell Culture Optimization
• Western Blotting
• Hplc
• Broad Molecular Biology
• Exceptional Project Management Skills
• Highly-Effective Communication Skills
• Electrophoresis & Blotting
• Gmp Environment
• Cross-Functional Team Management
• Clone Selection
• Project Management
• Management & Stakeholder Reporting
• Process Scale-Up & Transfer
• Critical Problem-Solving Skills
• Clinical Trials
• Standard Operating Procedures
• Molecular Cloning
• Validation
• Immunological & Biochemical Assays
• High-Level Data Analysis
• Immunoprecipitation Protocol
• Design of Experiment
• Bioreactors (Including Single-Use)
• Biologics Analytical Methods
• Molecular & Structural Biology
• Biotechnology
• Protein Purification
• Polymerase Chain Reaction (Pcr)
• Strong Analytical Skills
• Immunofluorescence
• Biosimilars & Follow-on Biologics
• Process Improvement / Fmea
• Protein & Peptide Chromatography
• Protein Expression
• R&D