V-Phuc Nguyen

WORK HISTORY

EDUCATION

ABOUT V-PHUC NGUYEN

Research scientist with an excellent productivity track record in R&D and regulatory industry R&D experience (immuno-oncology)* Expertise in flow cytometry (FACS) to analyze target or receptor expression and cytokine secretion, activation, proliferation or cytotoxicity, and other assays (SDS-PAGE, ELISA/ELISpot, DNA/RNA isolation and analysis, qPCR, gene cloning, siRNA/shRNA).* Proficiency with data acquisition and analysis using FACSDiva, FlowJo, LIMS, SDS, SoftMax, GraphPad. Participated in Electronic Lab Notebook to improve lab recordkeeping and regulatory compliance.* Hands-on experience to develop molecular (qPCR Mycoplasma and adventitious virus detection, CD19CAR) and cell-based assays (cell cycle, cytotoxicity)* Analyzed and compiled data for FDA Investigation of New Drug (IND) filing * Authored and edited SOPs leading to downstream process improvement, and for transfer to CMO for large scale manufacture * Expertise in handling cell lines and primary cells. Experience with human biological samples and infectious agents (BSL2/3 lab). Regulatory (GLP/GMP)* Excellent leadership and staff management: Trained and managed team of 8-10 analysts (BioReliance), 2 lab technicians and 3 students (UCI and NIH), 5 (1PhD scientist and 4 analysts)(Kite Pharma).* Team consistently achieved >95% on-time, accurate data delivery in performing various assays.* Acted as Subject Matter Expert (SME): 1) Author or Business Owner of multiple SOPs, 2) Resolved protocol deviations, 3) Effectively liaised with clients to resolve data issues, 4) Created databases to ensure testing compliance.* Drafted/Edited Certificate of Analysis (CoA) for lot release, and Quality Agreement as part of CRADA.* Detail-oriented, excellent time management and effective communication skills to complete simultaneous projects in cross-functional teams (AD, PD, QA, QE)