Tushar Rane

About Tushar Rane

Scientist/Engineer with extensive experience in developing bioanalyte sensing technologies. Workexperience includes being a key member of the core team that developed one of the world’s first fewcommercially available spatial molecular imagers (CosMx) from inception to a commercial product. Myacademic training and professional work spans a wide range of areas including microfluidic technology,optofluidic systems, molecular biology, instrument and data analysis software. I hope to leverage thebreadth of my experience to continue developing technology that enables new biomedical science andeventually makes a therapeutic impactMy visual CV (related to academic work only) can be found at http://tushardrane.weebly.com/

Experience

1. Principal Scientist

   Bruker Spatial Biology

   Jul 2024 — Present · Seattle, WA, US

   1) Developed advanced analytical and image processing pipelines in Python for large-scale CosMxRNA assay datasets, identifying raw data features important for data quality improvement, currentlybeing incorporated into commercial CosMx software.2) Standardized and implemented a comprehensive run quality report for CosMx subsystems, which isnow the primary tool in the troubleshooting pipeline for addressing customer complaints.3) Conducted experimental analysis that uncovered insights into factors driving CosMx RNA assayefficiency and specificity4) Developed a strategic proposal for a novel CosMx assay mode, leveraging existing hardware tosignificantly expand the platform\'s range of applications and market potential

Education

• Indian Institute of Technology, Bombay

  B. Tech, Mechanical Engineering

  2000 — 2004

• The Johns Hopkins University School of Medicine

  Doctor of Philosophy (PhD), Biomedical Engineering

  2007 — 2014

• The Johns Hopkins University

  MSE, Biomedical Engineering

  2005 — 2007

Skills

• R&D
• Multilayer Microfluidics
• Fluorescent Markers
• L-Edit
• Nanomaterials
• Matlab
• Computational Modeling
• Gel Electrophoresis
• Spectrophotometry
• Two-Photon Microscopy
• Pcr
• Solidworks
• Uv/Vis
• Microfluidics
• Experimentation
• Droplet Microfluidics
• Microfabrication
• Research
• Photolithography
• Data Analysis
• Nanotechnology
• Cell Culture
• Molecular Biology
• Fluorescence Microscopy
• Confocal Microscopy
• Nanoparticles
• Single Molecule Detection
• Lamp
• Life Sciences
• Sds-Page
• Fluorescence Resonance Energy Transfer
• Tem
• Confocal Fluorescence Spectroscopy
• Imagej
• Biomedical Engineering
• Polymerase Chain Reaction (Pcr)
• Nucleic Acid Hybridization
• Labview
• Soft Lithography
• Biomaterials