Tristan Miller-Cottle
Laboratory Operations Associate @Novartis
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WORK HISTORY
Laboratory Operations Associate @Novartis
Cross functional interdisciplinary role supporting the integration of all lab based functions.Ensures laboratory achieves and maintains pace of experimental regimens. Supports the development and characterization of commercial/clinical products and next generation processes.Prepares buffers for upstream and downstream experiments. Performs daily small equipment pre/calibrations. Executes daily sampling for the bioreactors including operation and setup. Upstream Supports Inoculation, Transfection and, Harvest of cells utilizing triple transfection of plasmids including AAV.Preforms adherent and suspension cell expansion and culture of HEK cells. Downstream operation of AKTA chromatography skids. Supports Purification, clarification, and,flocculation of downstream products. Logs pressure and turbidity readings. Pump setup and operation.Orates status, issues and actions to scientists and senior management.Manages Freezer and Warehouse inventory.
EDUCATION
Utica University
Bachelor's degree, Psychobiology/Neuroscience
ABOUT TRISTAN MILLER-COTTLE
I am looking for careers in genetics/biomedical sciences. I am a passionate research technician with advanced laboratory experience. Dedicated, & cooperative team player who is motivated to succeed & insightful.Exhibits leadership, innovative and intuitive mindset. Positive attitude including problem-solving capabilities & goal oriented. Thorough, precise, & consistent with my techniques. Organized, methodical, capable individual who is driven to excel. PROFESSIONAL DEVELOPMENTSenior Research Seminar: Genetic Engineering using CRISPR Cas9 (Utica University Senior Research Capstone Fall 2018-Spring 2019) Focused on stem cell research for regenerative medicine Investigated uses of genetic engineering in agriculture, bone & gene therapy, mental health, infertility, transgenics, and epigenetics.Drug Development/Pharmaceuticals characterization of clinical products and next generation processes.Process Development in Technology Research and Development (TRD)Manufacturing Science and Technology (MSAT) UNC School of Medicine Biochemistry & Biophysics Department Hematology Research Center key responsibilities focused on novel ways to study platelets and mechanisms of aggregation and adhesion. Utilizing various methods such as microscopy, aggregometry, flow cytometry, mean florescence intensity, flow chambers etc. Understanding platelet differentiation from Megakaryocytes and involved processes. Role of CD34 markers in stem cells and differentiation. Cell signaling processes involved in haemostasis and thrombosis and involved secretory proteins. Investigated mechanisms of hemostasis and thrombosis including platelet roles in inflammation, angiogenesis, tumor growth and metastasis. Cell biology and molecular biology of platelet/endothelial interactions. Working in a surgical plane utilizing sterile techniques. Platelet washing and separating blood from plasma and other experiments.
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