Timothy Baradet

About Timothy Baradet

SummaryAccomplished research professional with extensive industry experience in biomarker assay development, histopathology and companion diagnostic validation. ASCP certified in in Histotechnology (HTL) with a Qualification in Immunohistochemistry (QIHC). Recognized expert in applying molecular histopathology, spatial transcriptomics, and AI/ML digital pathology image analysis for research, translational studies & clinical trials. Able to multitask at a in a competitive & fast-paced environment with attention to detail. Committed to collaborative team work. Proficient in communicating study results for internal stakeholder and at external meetingsExperience:Biomarker Development & Validation for drug development, clinical trials and patient stratification utilizing multiplexed immunohistochemistry. molecular histology, digital pathology, image & data analysis and statistical analysis, and spatial transcriptomics, Highly experienced in multiple tissues and organs (brain, lung, liver, kidney, connective tissue) in human and animal tissues (murine, porcine)Research Specialties:Digital Pathology & Informatics – Experimental design & algorithms for chromogenic and multiplexed fluorescent immunohistochemical quantification. Quantitative analysis of biomarkers for evaluation & patient stratification. with image analysis software (Visiopharm, HALO, HALO AI, inform). Designing, implementing, and managing digital pathology applications and computing infrastructure covering the entire lifecycle of digital pathology projects. Develop AI/ML strategies for image analysis.Biomarker Development:General histology (tissue processing, embedding, paraffin microtomy and cryo sectioning. Routine & special stains. In situ hybridization (RNAscope & DNAscope). Automated immunohistochemistry using Leica Bond. Ventana Discovery, Biocare and DAKO autostainer platforms. Whole slide scanning with Zeiss AxioScan, Aperio & Akoya Phenoimager or similar)- Extensive practical experience in validation of antibodies and multiplex IHC panels. Record and organize in electronic lab notebooks and cloud computing assetsImaging: Multi-spectral and high content imaging & analysis (Vectra. Nuance, TRIO, Akoya Phenoimager). Bright-Field, Fluorescent, Confocal & 2-Photon Microscopy, Electron Microscopy (TEM, SEM, EDAX), Laser Capture Micro-dissection (LCM)Biochemistry: Electrophoresis, ELISA, Western Blotting, Column Purification, FPLC, HPLC, Light ScatteringMolecular Biology: PCR, RT-PCR, FISH, Blotting, RNAscope®, tissue culture

Experience

1. Scientist

   GSK

   Jul 2025 — Present

   Digital Pathology & Molecular Histopathology in support of complex disease model studies.

Education

• Drexel University College of Medicine

  Ph.D., Molecular Pathobiology

  1994 — 1999

• University of Pennsylvania

  M.S., Anatomy & Structural Biology

  1988 — 1993