Tarun Arya

WORK HISTORY

EDUCATION

ABOUT TARUN ARYA

Summary of the work during Postdoctoral Research• Biochemical assay development (ITC, FRET, MST, Biolayer Interferometry of RAS and RAF protein.• Fragment-based drug discovery strategy to identify novel inhibitors for the target protein. • Screening to identify leads for crystallography and further evolution into mature compounds. • Differential scanning fluorometry (DSF) for initial fragment screening of a fragment library and hit characterization. • Phage display assay to identify the potential binding partner of the target protein.Summary of the work carried out during PhD• Protein expression, purification and characterization. • Identification of the molecular basis of inhibitor selectivity• Hit identification by enzymatic assay.• Site directed mutagenesis.• Differential scanning fluorometry.• Co-crystallization of hit identified with the target protein.• X-ray crystallographySummary of the work carried out during M.Sc• Protein expression, purification and characterization of phycobilliprotein from cyanobacteria. • molecular modeling.• Bioinformatics.Summary of the work carried out during B.Sc• Protein expression, purification and characterization of ovine growth hormone from inclusion bodies. • western blot analysis of the active protein.• CD spectroscopy.