Syandan Chakraborty

WORK HISTORY

EDUCATION

SKILLS

ABOUT SYANDAN CHAKRABORTY

Six years of industry experience and two years of postdoctoral training specializing in genome editing, CRISPR screening, synthetic biology, gene delivery, cellular reprogramming and differentiation. • Expertise in drug discovery shaped by extensive clinical knowledge and bioengineering skills. • Published 14 articles with over 1,800 citations, presented at eight international conferences, filed one patent application, and obtained two research grants. • Engaged efficiently with cross-functional teams and CROs, mentored scientists, taught graduate-level courses, and assisted startups.Research Skills:• Cell culture: Culture of primary cells, cell lines, iPSCs, and embryonic stem cells in 2D and 3D culture systems (e.g, nanofibers, hydrogels, AggreWell, hanging droplets, and microfluidic encapsulation). Transgenic cell enrichment and isolation by single-cell cloning, antibiotic selection, and FACS. • Genome editing and gene delivery systems: CRISPR/Cas9, zinc finger nuclease, homology-directed repair, degron tagging, lentivirus, piggyBac transposons, chemical transfection, and nucleofection. • Molecular and synthetic biology techniques: Molecular cloning by restriction digestion, Gateway, In-Fusion, Gibson assembly, Cre-LoxP recombination, and TOPO-TA. • Assays and laboratory techniques: Flow Cytometry, Immunocytochemistry (ICC), fluorescence and confocal microscopy, Incucyte live-cell imaging, Operetta high content imaging, electron microscopy, Bravo and Combi automated liquid handlers, RT-qPCR, PCR-RFLP, ddPCR, microarray, Sanger sequencing, NGS, pooled and arrayed CRISPR screens, mismatch cleavage assays, cell proliferation assay, ELISA, western blot, luciferase assay, and chromogenic assay. • Computing and data analysis: UCSC Genome Browser, BLAST, PlasmaDNA, SnapGene, ICE and OutKnocker analysis, intermediate-level R, MATLAB, GraphPad Prism, NIS Elements, and ImageJ.