Swar Christian

Scientist Ii Rna @4basebio

Cambridge, GB

IVT (In vitro Transcription) of synthetic and plasmid DNA, performing its QC analysis. Nucleic acid quantification through nanodrop and Broad Range Qubit assay, LiCL2 based RNA precipitation, Plasmid Linearization, Linearized DNA precipitation • Dot Blot for dsRNA characterization, native, denaturing, UREA PAGE gel electrophoresis for RNA quality and integrity. •PCR, Gel excision+Gel elution, Cloning, Bacterial transformation, Colony PCR for template generation followed by its IVT. • Oligo-dt pulldown for poly-A tail analysis of IVT generated RNA and mini-cellulose purification for its reduction in ds-RNA content, RT-PCR for generating cDNA from IVT RNA followed by sequencing through MinION (Oxford Nanopore)•pegRNA generation through enzymatic approach, followed by its QC analysis mainly through Denaturing PAGE, Fragment Analyzer, RT-PCR and sequencing approach.• Snapgene for RNA and plasmid analysis, ELISA for dsRNA quantification and PCR for dsRNA synthesis. RNA integrity, size Determination, structural analysis and poly A length analysis using CE methods like Qiaxcel and Agilent Fragment Analyzer 5200• Media, buffer, reagent preparation, following good laboratory practices, routine oral presentations on related research articles, weekly meetings and updating lab work routinely in electronic workbook.