Supriya H S

WORK HISTORY

EDUCATION

ABOUT SUPRIYA H S

Protein and enzyme purification using AKTA pure, AKTA pilot, AKTA process and NGC systems. • Protein and enzyme purification by chromatography techniques like MMC, Ion-exchange, Affinity, HIC methods. • Diafiltration and Ultrafiltration using different MWCO TFF cassettes. • TFF filtration study optimization. • Involved in Depth filtration studies for different enzymes. • Involved in GMP batch study and scale up protocol of PEG-GCSF. • Absolute and Nominal filtration studies for different buffers and recombinant proteins. • Preparation of Study protocol and SOP. • Handled Change control and Deviations.*Cloning of different vector systems such as Alpha HC, HC, LC, pUC19 and pPic9. •Thorough in recombinant Plasmid isolation and characterization by restriction digestion. •Designing complex rDNA sequences, primers and amplification using PCR. •Handling of bacterial cultures like DH5α. Handling of yeast cultures like pink Pichia pastoris. •Protein purification by Affinity chromatography and Ion exchange chromatography. •Protein detection by SDS-PAGE followed by Western blotting. •Protein quantification by UV-spec, Agilent Spectrophotometer. •Bio-informatics tools such as Pymol, Protein Blast, DNA alignment Complete end-to-end in-house and reference material inventory management with CDSCO compliance. Ensuring laboratory’s EHS compliance. Ensuring laboratory’s QMS compliance. Procurement of laboratory consumables using SAP. Ensuring proper functioning of all stability chambers, cooling cabinets, freezers and ultra-low temperature deep freezers in the laboratory. Cross-functional communications with R&D, quality, production, regulatory, operations, program management, commercial, central engineering, supply chain, safety and finance teams. Stability data compilation & trending. Tracking of stability study & raw material Out of Trend (OOT) incidents. Tracking of equipment Out of Calibration (OOC) incidents. Tracking of system deviations.