Sunita Subramanian
Senior Scientist at Nabla Bio, Inc.
- Role
- Senior Scientist at Nabla Bio
- Location
- Boston, MA, US
- LinkedIn followers
- 500 followers
About Sunita Subramanian
I am a biochemist with sustained experience in Protein Structural Biology and X-ray Crystallography. My research has focused on furthering the understanding of biomolecules that are implicated in crucial biological processes, such asa. Enzymes involved in conferring bacterial antibiotic resistanceb. Endonucleases used as tools in genome engineeringc. Human protein kinases critical to the innate immune responsed. Human HIV restriction factors that block infectious pathwayse. Ribosomal complexes to elucidate fundamental steps in protein translationProfessional Summary: I have performed detailed characterizations of a diverse array of macromolecular targets to illuminate the structural basis for their function. I am passionate about applying my extensive research training to science communication, as well as research and product development and biochemical diagnostics. Technical expertise: Cloning, Recombinant protein design and expression, scale-up and purification using FPLC systems, Protein engineering, High Throughput crystallization trials, High resolution structure solution (crystallography), Isothermal Titration Calorimetry, Radiometric Assays, Mass Spectrometry, Electrophoretic Mobility Shift Assays, Circular Dichroism Spectroscopy, Western Blot, Immunoprecipitation, Differential Scanning Fluorimetry, Bioluminescent Assays, Real-time PCR, Antibody Purification, Mammalian Cell Culture, Virus production and purification
Experience
Senior Scientist
Feb 2021 — Present
Education
National University of Singapore
Doctor of Philosophy (PhD), Macromolecular Protein Crystallography
2004 — 2008
Anna University Chennai
Bachelor of Technology (BTech), Industrial Biotechnology
1999 — 2003
Skills
- Life Sciences
- Binding Assays
- Hplc
- Methylation
- Polymerase Chain Reaction (Pcr)
- Scientific Writing
- Protein Purification
- Mentoring
- High-Performance Liquid Chromatography (Hplc)
- Maldi-Tof
- Molecular Biology
- Protein Chemistry
- Protein Expression
- Fplc
- Isothermal Titration Calorimetry
- Cell Culture
- X-Ray Crystallography
- Molecular Cloning
- Rna Biology
- Enzyme Assays
- Protein Engineering
- Biochemistry
- Phosphorylation
- Protein Crystallization
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