Subbanna Shivakumar
Assistant Research Scientist @Research Foundation for Mental Hygiene, Inc.
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WORK HISTORY
Assistant Research Scientist @Research Foundation for Mental Hygiene, Inc.
Neurochemistry, Neuropharmacology, epigenetics & mice Behavioural analysis.My role in brief Alcohol exposure can affect brain development, leading to long-lasting behavioral problems, including cognitive impairment, which together is defined as fetal alcohol spectrum disorder (FASD). However, the fundamental mechanisms through which this occurs are largely unknown. In this study, we report that the exposure of postnatal day 7 (P7) mice to ethanol activates caspase-3 via cannabinoid receptor type-1 (CB1R) in neonatal mice and causes a differential expression in DNA binding proteins G9a, CREB, HDAC DNMT3 MeCP2 levels. The developmental expression of these proteins in mice is closely correlated with synaptogenesis and neuronal maturation. It was shown that ethanol treatment of P7 mice decrease/enhance mRNA levels. The genetic deletion of CB1R prevented, and administration of a CB1R antagonist before ethanol treatment of P7 mice which inhibited caspase-3 activation. Additionally, it reversed the loss of these protein. The inhibition of caspase-3 activity prior to ethanol administration prevented ethanol-induced loss of DNA binding proteins, epigenetic regulation, long-term potentiation (LTP), spatial memory deficits and activity-dependent impairment of several signaling molecules, including in adult mice. Collectively, these results reveal that the ethanol-induced CB1R-mediated activation of caspase-3 degrades the DNA binding protein in the P7 mouse brain and causes long-lasting neurobehavioral deficits in adult mice. This CB1R-mediated instability of these proteins during active synaptic maturation may disrupt synaptic circuit maturation and lead to neurobehavioral abnormalities, as observed in this animal model of FASD.
EDUCATION
Govt Sri mahadeshwara College Kollegal karnataka India
Bachelor's Degree, Biochemistry, Botany & Zoology
Nathan kline institute
Postdoctoral Assistant research Scientist, Neurobiology, Neuropharmacology and Behavior
Department of Biotechnology, University of Mysore, Manasagangotri, Mysore India
Master of Science (M.Sc.), Biotechnology
Govt M.G.S.V Jr College Kollegal Karnataka India
High School
SDA School Kollegal Karnataka India
Primary & Middle School
Department of Biotechnology, University of Mysore, Manasagangotri, Mysore India
Doctor of Philosophy (Ph.D.), Angiogenesis in Cancer and RA
Vijaya Teachers College, Jayanagar, Bangalore
Bachelor of Education (B.Ed.), Chemistry & Biology
Govt. Sri Mahadeshwara College, Kollegal
Biochemistry, Botany & zoology
ABOUT SUBBANNA SHIVAKUMAR
Highly focused innovative research scientist with more than 12 years of experience in…
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