Steven Schultze

Research Technician Ii @University Of Louisville School Of Medicine

Louisville, KY, US

Genetic Sequencing Technician October 2022 – Present Watson Lab University of Louisville, Louisville, KY • Analyzed genetic sequences in immune protein regions• Integrated targeted genetic sequencing data with quality control data to improve and optimize processes• Collected and analyzed quality control data for ongoing troubleshooting and process monitoring• Analyzed genetic sequence data in highly complex regions of the genome• Integrated label printers and other new technologies to improve lab efficiency• Performed unsupervised cluster analysis on genetic sequencing data • Completed master of science while working full time• Independently organized and planned overlapping multistep scientific protocolsResearch Animal and Laboratory Technician July 2021 – October 2022 Bodduluri Lab UofL Microbiology and Immunology, Louisville, KY • Independently managed 13 genetically distinct colonies of transgenic research mice• Routinely isolated microbial DNA from fecal samples for NanoPore DNA/RNA sequencing• Performed genotyping PCR amplification reactions for multiple strains of gene knockout mice• Purified and prepared samples for 16S ribosomal RNA sequencing analysis of microbiome samples• Practiced proper chemical safety and sterile technique at the benchtop and within biosafety cabinets• Conducted daily lab duties in BioSafety Level (BSL) 2 environments and Animal Biosafety Level(ABSL)2• Provided succinct and organized updates regarding status of multiple research animal colonies• Regularly attended seminars and guest speaker lectures to further my education and knowledge • Maintianed inventory and oversaw ordering of supplies for daily lab operational needs