Steven Mullenbrock

About Steven Mullenbrock

Utilized a systems biology approach that combined gene expression analysis, computational analysis, and molecular/cell biology techniques to identify transcriptional programs downstream of growth factor induced signaling pathways in mammalian cell lines. Currently using similar approaches with in vivo animal and in vitro cellular models to identify critical pathways involved in fibrotic disease (IPF and SSc).Specialties: Gene expression analysis, signal transduction, transcriptional regulation, mammalian cell culture, siRNA and plasmid transfection, real time PCR, western blots, subcloning (plasmid, bacterial work), RNA/DNA/plasmid purification, chromatin immunoprecipitation (ChIP), general cell/molecular biology techniques.Experience using Partek Genomics Suite, GENE-E (Broad Institute), GraphPad Prism, Integrated Genomics Viewer, gene ontology/pathway analysis (Ingenuity, Metacore, DAVID)

Experience

1. Principal Scientist

   Cell Signaling Technology (CST)

   Feb 2023 — Present · Danvers, MA, US

Education

• Boston College

  B.S., Biology

  1998 — 2002

• Boston University

  Ph.D., Biology (Cell and molecular biology)

  2002 — 2011

Skills

• Sds-Page
• Apoptosis
• Polymerase Chain Reaction (Pcr)
• Tissue Culture
• Western Blotting
• Microscopy
• Rt-Pcr
• Mammalian Cell Culture
• Real-Time Pcr
• Chromatin Immunoprecipitation
• Molecular Cloning
• Reverse Transcription Polymerase Chain Reaction (Rt-Pcr)
• Transcriptional Regulation
• Real-Time Polymerase Chain Reaction (Qpcr)
• Bacterial Transformation
• Gel Electrophoresis
• Luciferase Assay
• Dna Extraction
• Sirna
• Purification
• Gene Regulation
• Pcr Primer Design
• Gene Expression Profiling
• Reporter Gene Assays
• Cell Signaling
• Qpcr
• Cell Culture
• Cell
• Cell Biology
• Emsa
• Molecular Biology
• Transfection
• Signal Transduction
• Pcr
• Western Analysis
• Rna Isolation