Sourabh Pujari
Graduate Research Assistant @Northeastern University, Bouvé College Of Health Sciences
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WORK HISTORY
Graduate Research Assistant @Northeastern University, Bouvé College Of Health Sciences
Boston, MA, US
Performed transfections on the BOSCH 23 cells using alpha-7 and 5HT3 Chimera to check expression of alpha 7 receptor. Conducted flow cytometry experiments to measure transfection efficiency. Optimized the DNA-to-XtremeGene ratio to achieve maximum transfection while minimizing cytotoxic effects. Analyzed the impact of Ric3 and TMEM35 (NACHO) on the expression of alpha 7 receptor. Utilized Western blotting techniques to assess the expression levels of chaperons Ric3 and TMEM35. Incorporated Gel Exclusion Chromatography Technique in Iodination Process, resulting in a 15% increase in efficiency. Researched cytokine-induced cellular responses in HEK-Blue cells by using IL6 and TNF-alpha. Studied cellular signaling pathways by analyzing alkaline phosphatase secretion in HEK-BLU cells.
EDUCATION
Northeastern University
Master's degree, Biotechnology
KIT's College of Engineering Kolhapur (Empowered Autonomous)
Bachelor of Technology - BTech, Biotechnology
Swami Vivekanand College Kolhapur
12 th, Science
ABOUT SOURABH PUJARI
Graduate student in Biotechnology at Northeastern University. Proficient in mammalian cell culture, flow cytometry, western blotting, qPCR, chromatography, and autoradiography Experienced with lab tools such as Fluorescent Microscope, Flow Cytometer and Gamma Counter. Familiarity with software applications: JMP, Image-J, GraphPad Prism, ChimeraX, ApE. Strong professional skills in biochemistry, microbiology, and molecular biology.
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