Songyu Wang

About Songyu Wang

Experienced biochemist specializing in purification and characterization of recombinant…

Experience

1. Principal Scientist

   Amgen

   Jul 2023 — Present

   Protein Design and Engineering group in Discovery Protein Science

Education

• National University of Singapore

  Doctor of Philosophy (Ph.D.), Cell Biology and Genetics

  2005 — 2011

Skills

• Immunostaining and Live-Cell Imaging
• Test Expression to Determine Optimal Tags and Detergent
• Nanobody Generation
• In Vitro Er Network and Nuclear Assembly Using Xenopus Extracts
• Liposome Preparation and Flotation
• Size Exclusion
• Affinity Chromatography Using His, Gst, Sbp and Mbp Tags
• Genetic Screening in Budding Yeast
• Life Sciences
• Affinity Purification of Antibodies
• Protein Purification of Tagged and Untagged Membrane and Cytosolic Proteins
• Ion Exchange
• Sds-Page
• Wide Field or Confocal Fluorescence Microscopy
• Purifying Protein Domains as Antigens for Antibody Raising
• Western Blotting
• Biochemistry
• Membrane Protein Reconstitution
• Pulse-Chase Analysis with Radioisotope 35s
• Cell Culture
• Dynamic Light Scattering
• Preparation of Crude and Fractionated Xenopus Egg Extracts
• Isolation of Sperm Chromatin From Male Frogs
• Detergent Removal with Different Detergent Removable Methods
• Digital Image Processing
• Protein Labeling Using Biotinylation Reagents, or Sortase Methods
• Sucrose Gradient Centrifugation
• Hydrophobic Interaction Chromatography
• Protein Purification From Bacteria, Yeast, Insect, and Mammalian Cells
• Imagej
• Protein Purification
• Polymerase Chain Reaction (Pcr)
• Biophysical Assays
• Elisa
• Flow Cytometry
• In-Gel Fluorescence Scanning Using Li-Cor
• Fplc (the Akta System with the Unicorn Software)
• Protein Labeling Using Amine or Sulfhydryl-Reactive Reagents
• Gene Cloning (Restriction, Gibson Assembly),Gene Block,Site-Directed Mutagenesis
• Immunoprecipitation