Sohinee Bhattacharyya.

About Sohinee Bhattacharyya.

SUMMARYPh.D-trained immune oncology investigator with over 8 years of experience in working with clinical patient samples, creating and utilizing genetically engineered mouse and cell models (primary cells, cancer cells, and organoids) to investigate dysregulated oncogenic signaling in cancers. Specialized in using small-molecule and CRISPR/Cas9-based strategies to inhibit cancer growth by targeting oncogenic signaling, metastasis, EMT, cancer metabolism. Proficient in cancer signaling pathways (KRAS, PI3K, MAPK, NFKB, MYC) and skilled in using molecular and cellular tools to investigate tumor immune microenvironment and develop rational therapeutic strategies. Specializing in animal models of disease, along with complex data analysis and interpretation.HIGHLIGHTS- Successfully led multiple in vivo projects from inception to completion, resulting in high-impact publications in Nature Cancer, Journal of Experimental Medicine, Cancer Discovery- Developed and executed comprehensive in vivo studies, using orthotopic models, and patient-derived cell lines- Designed small molecule pharmacological inhibitor screens and CRISPR and lentivirus-based gene editing techniques in animal models to investigate tumor-immune interactions- Demonstrated expertise in communicating complex scientific findings through grants, and presentations- Extensive experience in training students, and working in fast-paced, multi-cultural, and dynamic environments.SPECIALTIES- Expertise in in vivo models of cancer, including subcutaneous, orthotopic, xenograft, transgenic models- Experienced in designing small molecule pharmacological inhibitor screens, elucidating mechanism of action, and target validation- Expertise in confocal microscopy- Designing and analyzing multi-color flow cytometry panels, using FACS to isolate pure cell populations from tissue samples- Experienced in isolating and establishing in vitro model systems from mouse tissues and human patient samples. TECHNICAL SKILLS- In vivo mouse models (xenografts, orthotopic, PDXs, transgenic), anti-tumor assessment- Mammalian cell culture- Immune cell isolation and expansion, multicolor flow cytometry to analyze immune subpopulations - Gene editing (CRISPR/lentivirus vector-based gene targeting methods)- ELISA- Western blotting, qPCR, Primer design, PCR, and Agarose gel electrophoresis- DNA isolation, RNA isolation, High throughput sequencing- Small molecule screening and target validation- Immunohistochemistry, Confocal Microscopy- GraphPad Prism I SigmaPlotI Flow Jo I ImageJ

Experience

1. Senior Scientist

   Pfizer

   Feb 2025 — Present · Seattle, WA, US

   In Vivo Pharmacology, Drug Discovery and Development, Pre-Clinical Oncology Research

Education

• UNMC

  Doctor of Philosophy (Ph.D.), Pathology

  2009 — 2015

• University of Calcutta

  Master of Science (M.S.), Microbiology

  2006 — 2008

• University of Calcutta

  Bachelor of Science (B.S.), Microbiology

  2003 — 2006