Sidney Hsin-Kang Hsieh
Associate Principal Scientist at Merck
- Role
- Associate Principal Scientist at Merck
- Location
- Boston, MA, US
- LinkedIn followers
- 500 followers
About Sidney Hsin-Kang Hsieh
20 years of research experience in Biotechnology and Pharmaceutical IndustrySKILLS Science- Primary cell culture: super cervical ganglia, dorsal root ganglia, pure astrocyte, microglia, cortical neurons, hippocampal neurons, cerebellum granule neurons, macrophage, brain slice culture, splenocytes, isolated NK cells, isolated T cells, tumor cell culture- Animal work: Mice, Rats, Chicks - Biostatical analysis: Prism, Datasis- Proficient in the use of many molecular biology techniques: Western/ Northern/ Southern blotPCR/ RT-PCR/ qPCRICCIHCIn-cell western blotAnimal behavioral studyFluorescence and Confocal microscopyTransformation/ TransfectionLuciferase assayProliferation assayConditional knockout cloningSurgical techniquessiRNA design and overexpression.ELISA/ AlphaLISA/ MSD/ HTRFFACS and Flow CytometryHigh Content Imaging SystemHigh-Throughput Cell based drug screening assaysMicroscopy Imaging Analysis Cell Proliferation Assay/ Cell Viability Assay/ Cytotoxicity AssayChemotaxis Assay/ Phagocytosis AssayCalcium Mobilization Assay (FLIPR)TranscriptomicsProteomicsComputer- Molecular biology and DNA analysis software: DNAsis, DNAman, BLAST- Imaging analysis: Photoshop, Illustrator, NIH-ImageJ, Metamorph, InCell- Quantitative analysis: Prism, FlowJo
Experience
Associate Principal Scientist
May 2024 — Present · Cambridge, MA, US
Education
McGill University
B.Sc, Biochemistry & Immunology
1999 — 2002
McGill University
M.Sc, Neuroscience
2003 — 2006
Skills
- Research
- Reverse Transcription Polymerase Chain Reaction (Rt-Pcr)
- Protein Kinases
- Microscopy
- Real-Time Pcr
- Inflammation
- Assay Development
- Primary Cells
- Cell Culture
- Neurodegenerative Disease
- Immunoassays
- Alzheimer's Disease
- Elisa
- Biotechnology
- Fluorescence
- Polymerase Chain Reaction (Pcr)
- Life Sciences
- Cell Based Assays
- Drug Development
- Molecular Cloning
- Pcr
- Biochemistry
- Drug Discovery
- Neuroscience
- Western Blotting
- Real-Time Polymerase Chain Reaction (Qpcr)
- Immunocytochemistry
- Synergies
- Transfection
- Signal Transduction
- Molecular Biology
- Fluorescence Microscopy
- Target Identification
- Sirna
- Flow Cytometry
- Primary Cell Isolation
- Immunohistochemistry
- Qpcr
- Cell Signaling
- Cell Biology
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