Siddhesh Lad

About Siddhesh Lad

With over 13 years of dedicated experience in the biotechnology sector, I am a highly…

Experience

1. Scientist

   Biogenomics

   Jun 2022 — Present · Thane, IN

   Oversee critical fed-batch E. coli-based fermentation processes, maintaining optimal conditions for high-yield protein production.• Manage the seamless scale-up of fermentation processes, ensuring successful transitions from laboratory-scale experiments to full production capacity.• Supervise all stages of protein purification, implementing stringent quality control procedures to uphold product integrity.• Operate within a cGMP-compliant production facility, managing automated 1kL fermenters and overseeing protein purification workflows.• Analyze research, testing, and quality control reports to consistently meet quality standards, operational efficiency, and production timelines.• Spearhead the creation and revision of Standard Operating Procedures (SOPs) and prepare Batch Manufacturing Records (BMRs) and Master Manufacturing Formula (MMF) documentation, ensuring compliance and operational consistency.• Lead the management of incident reports, proactively initiating planned and unplanned deviations, and implementing Corrective and Preventive Actions (CAPA) for continuous improvement.• Contributed to clinical trial validation and technology transfer batches for Filgrastim and Insulin Aspart, ensuring accurate and consistent outcomes during fermentation and inclusion body isolation processes.• Operate and troubleshoot continuous centrifugation and cell disruptor systems (Homogenizer), resolving operational challenges to ensure minimal downtime and maximum efficiency.• Collaborate effectively within cross-functional teams to resolve challenges and consistently meet production targets.

Skills

• Elisa
• Hplc
• Biotechnology
• Pcr
• Molecular Biology
• Cell Culture
• Life Sciences
• Pharmaceutical Industry
• Biochemistry
• Microbiology