Siddhesh Baraskar
|| Genetic Engineering || Metabolic pathway engineering || Strain Development || Recombinant protein Expression and Purification || Antibody Phage Display || Analytical Method Development ||
- Role
- Scientist at Yashraj Biotechnology Ltd
- Location
- Mumbai, MH, IN
- LinkedIn followers
- 500 followers
About Siddhesh Baraskar
Strategy Planning and Execution of Projects including Recombinant Antibody Clone Discovery using Phage Display, Vector development, Expression and Purification of Commercially important proteins in E.coli and Bacillus, Strain Development and Metabolic Pathway Engineering for Expression Optimizations, Fermenter Study in E.coli and Bacillus
Experience
Scientist
Nov 2020 — Present · Mumbai, IN
Key Roles :1.Project Conceptualization, Strategy design for Construction and characterization of camelid single domain antibody phage display library.2.Development of Bio panning and Clone Screening process for 6 Industrially important Cancer Antigens.3.DNA Sequencing and Chromatogram Analysis.4,Site Directed Mutagenesis using overlap PCR.5.E.coli Vector Development for large scale Expression of Lead antibody candidates.6.Refolding and Purification of Recombinant proteins from Inclusion Bodies and Periplasmicspace.7.Method Development for Conjugation of Proteins.8.Team management, Data analysis, Process optimizations, updates to senior management.9.New R&D Facility setup as per ISO 13485 & 9001.10.Inventory and Instrumentation In charge, responsible for maintenance and procurement.
Education
Dr. D. Y. Patil School of biotechnology and bioinformatics
M.tech integrated
2014 — 2019
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