Shreshth Gupta
Diagnostic Assay & LFA Development | Protein Expression & Purification| RT-PCR & Molecular Diagnostics | Antibody & Protein Engineering | M.Tech Biotechnology | MANIT, BHOPAL | AIIMS, BHOPAL
- Role
- Associate Research Scientist at Nextgen In-Vitro Diagnostics Pvt. Ltd
- Location
- Gurugram, HR, IN
- LinkedIn followers
- 500 followers
About Shreshth Gupta
Healthcare professional experienced in Rt-PCR(QS-5, CFX), Primer designing & Invivo…
Experience
Associate Research Scientist
Nextgen In-Vitro Diagnostics Pvt. Ltd
Sep 2023 — Present · Manesar, IN
Developed and transferred technologies for DNA/RNA extraction kits (spin column & magnetic bead-based) from diverse sample types.Designed and optimized RT-PCR/qPCR assays for infectious diseases (HPV, Flu, RSV, COVID-19, H1N1, Sepsis) with improved sensitivity and specificity.Engineered and purified proteins such as DNA Polymerase, Proteinase K, and RNase A in yeast and bacterial systems.Developed and validated Lateral Flow Assays (LFAs) for major infectious diseases (HIV, HBsAg, HCV, Malaria, Syphilis, Dengue NS1, IgG/IgM).Expertise in cloning, transformation (chemical & electroporation), competent cell preparation, and antibody conjugation techniques.Beyond technical skills, I bring strengths in workflow optimization, SOP documentation, data analysis, and cross-functional collaboration, with a strong commitment to quality, reproducibility, and regulatory compliance.
Education
Dr. A.P.J. Abdul Kalam Technical University
Bachelor of Technology - BTech
2017 — 2021
Maulana Azad National Institute of Technology
Master of Technology - MTech
2021 — 2023
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