Shijie W.

About Shijie W.

Postdoctoral scientist with 8+ years of experience specializing in neurodegenerative…

Experience

1. Postdoctoral Researcher

   Gladstone Institutes

   Jul 2020 — Present · San Francisco, CA, US

   Current research focus on leveraging automated robotic microscopy and deep learning to model and predict cell fate under pathological stress in neurodegenerative diseases and cancer.Understanding Neurodegeneration Diseases:1. Developed nuclear biomarkers and deep learning models to predict neuron cell death2. CRISPR Screening of Parkinson\'s Disease Targets: Led a genome-wide CRISPR screen to identify survival modulators in Parkin-deficient neurons.3. Understanding Tau Pathology in Neurodegeneration: Leveraged longitudinal imaging and deep learning to identify Tau-dependent cellular dynamics; Engineered fluorescent biosensors to study Tau turnover and neurotoxicity, and enable real-time tracking of neurodegenerative pathways; Use biosensor-integrated longitudinal imaging datasets and deep learning to understand the sub-cellular changes after Tau seeding in neuron, uncovering mechanistic insights into neurodegeneration.4. Down Syndrome (DS): Characterize AD-related phenotypes using DS patient iPSC-derived neurons and organoids models; Generate stable CRISPRi iPSC lines for mechanistic validation.Technology Development- Co-developed a next-generation longitudinal imaging platform enabling light-based recording, perturbation, and isolation of individual cells, reducing inter-well variability and enabling precise optogenetic control- Integrating deep learning models to automate cell selection and analysis, creating a scalable framework for multi-modality projects- Understanding cancer drug resistance in cancer cells: Discovered biomarker for drug-resistant NSCLC, generated large dataset to implement deep learning to predict drug resistant cells prior to the drug treatment, developed methods to isolate drug-resistant cancer cells at single cell level, enabling unbiased cell selection strategies and multi-omics methods to understand the underlying mechanism of cancer cell drug resistance.

Education

• University of Alabama at Birmingham

  Doctor of Philosophy - PhD

  2015 — 2019

• Shenzhen University

  Bachelor of Science (BS)

• University of Illinois at Chicago

  Master of Science (MS)