Shawna Henry

WORK HISTORY

EDUCATION

ABOUT SHAWNA HENRY

Rigorous research scientist with over eight years of experience leveraging cell/molecular and biochemical techniques to investigate neurodegenerative disease therapeutics. Over two years\' experience in a biotech setting performing target deconvolution and mechanism of action studies for a small molecule program aimed at improving lysosomal function. Self-motivated, curious, and team-oriented scientist adept at collaborating on cross-disciplinary teams. Core Skills and Techniques: • Target ID: photoaffinity labeling• Copper-mediated click chemistry • Affinity Purification • HiSPECS secretome analysis• Western blot (LI-COR) • ELISA• Co-Immunoprecipitation • CETSA• Cytotoxicity assays (MTS, LDH)• Sucrose gradient organelle isolation• Live cell internalization assays• Molecular cloning (Gibson, Site-directed mutagenesis, PCR) • siRNA knockdown• Plasmid transfection • Lentivirus production and infection• Protein purification• Mammalian cell culture (with SILAC)• Primary murine neuronal culture• Immunocytochemistry• Confocal microscopy (Zen, Imaris, ImageJ)• Statistical analysis (Graphpad Prism)• Microsoft (Word, Excel, PowerPoint, Outlook)Contact: s••••••••@gmail.com