Shaunak Uplekar
Director, Upstream Process Development at KBI Biopharma
- Role
- Director, Upstream Process Development at KBI Biopharma
- Location
- Raleigh-Durham-Chapel Hill, NC, US
- LinkedIn followers
- 500 followers
About Shaunak Uplekar
A Bioprocess technologist, working on cell culture that produces monoclonal antibody (mAb). Studies mAb titer and quality aspects such as glycosylation profiles as a function of various cell culture conditions. Overall goal is to pursue a career in bioprocessing research and development.TECHNICAL SKILLSCell culture:Experience with culturing, passaging, freezing and thawing of mammalian cells such as murine hybridoma, CHO and fibroblast cell lines, Cell culture in bench-top bioreactors, minibioreactors and spinner flasks, Kla measurements, Scale down, Cell viability assays and microscopic techniques.Chromatography: Preparative chromatography (Affinity, Pseudo-affinity, & Ion-exchange), preparative column packing and testing, covalent chemistries to attach ligands to base matrices to make novel chromatographic matricesAnalytical: HPLC (UV-Vis) for proteins, HPAEC (PAD) for glycans and monosaccharides, SEC, MALDI TOF, Fluorescence spectrophotometer, UV-Vis Spectrophotometer, Optical sensor measurements for DO, pH and CO2Protein Chemistry: Glycosylation analysis, ELISA, SDS-PAGE, Gel Imaging and documentationGeneral Laboratory Techniques: General microbiology, Lyophilization, Dialysis, Sugar analysis, YSI, Kjeldahl analysis, membrane filtrationOthers: Statistical design of experiments (DOE), Certificate in ‘Biochemical Regulatory Engineering’
Experience
Director, Upstream Process Development
Dec 2024 — Present
Education
University of Maryland Baltimore County
PhD, Chemical Engineering
2007 — 2013
Institute of Chemical Technology (Formerly UDCT) , Mumbai, India
M. Tech, Bioprocess Technology
2005 — 2007
Institute of Chemical Technology (Formerly UDCT) , Mumbai, India
B. Tech., Chemical Technology (Specialization in Food Engg)
2001 — 2005
Skills
- Filtration
- Hplc
- Monoclonal Antibodies
- Ion Exchange
- Bioreactor
- Sds-Page
- Maldi-Tof
- Design of Experiments
- Elisa
- Chromatography
- Spectrophotometry
- Chemical Engineering
- Cell
- Fluorescence
- Protein Purification
- Cell Culture
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