Sean Cater

About Sean Cater

Skilled molecular biologist with extensive experience in high-throughput DNA build workflows, nucleic acid manipulation, NGS assays and leading successful project teams. 20 years of biotechnology experience, including over 15 years of turning ideas into tangible products. Adaptable and proficient at synthesizing information as part of a cross-functional team. Adept at problem identification and resolution, risk mitigation, utilization of all tools at my disposal, and creation of new tools when needed.Proficiencies: Multiplex PCR, Droplet digital PCR (ddPCR) and RT-PCR, qPCR, DNA/RNA sample prep, Illumina NGS library prep, DNA build (Gibson assembly, Golden Gate assembly, CRISPR), Bacterial strain build (protoplasting, transformation, conjugation, transposon mutagenesis, genome shuffling), automated sample handling (Bravo, Tecan Evo, Tecan Fluent, Hamilton STAR, Echo, I.DOT, QPix), gel electrophoresis, Western blotting, site-directed mutagenesis, ligations, DNA synthesis, conjugation chemistry, protein purification, cell culture, FACS, microscopy, HPLC, LC-MS, mirofluidic control, photolithography and PDMS device prototyping. Python, LIMS, AutoCAD, JMP, Spotfire, Chemdraw, Microsoft Office, GSuite, Smartsheets

Experience

1. Senior Scientist

   Adaptive Biotechnologies Corp.

   Feb 2023 — Present · Seattle, WA, US

   Designing high-throughput DNA build workflows for construction of large-scale immune receptor libraries using Gibson Assembly and Golden Gate Assembly- Managing project teams to collect large-scale immune-repertoire NGS datasets for various T-cell and B-cell diagnostic and therapeutic projects- Creating high-throughput automation-based laboratory workflows and analysis schemes for multiplex PCR and NGS assays for T-cell and B-cell characterization- Developing NGS and qPCR assays as molecular readouts of immunotherapy efficacies for multiple species- Modeling potential assay variations to improve designs and efficiencies- Mining immune sequencing databases to help characterize assay performance and direct the expansion of assay designs and utilization- Building analysis and bioinformatics software - Immune sequencing assay design, development and analysis

Education

• University of California, Berkeley

  MA, Molecular and Cell Biology

  2005 — 2007

• The University of Texas at Austin

  Bachelor of Science (BS), Biochemistry

  2000 — 2005

Skills

• Biochemistry
• Molecular Biology
• Protein Purification
• Cell Culture
• Pcr
• Polymerase Chain Reaction (Pcr)