Saurabh Mehta
Senior Research Scientist-protein @Isolere Bio, A Donaldson Life Sciences Business
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WORK HISTORY
Senior Research Scientist-protein @Isolere Bio, A Donaldson Life Sciences Business
Raleigh-Durham-Chapel Hill, NC, US
Design, plan, execute and interpret complex recombinant protein purification protocols leading to process development and optimization for purification of proprietary proteins for use in the non-chromatographic purification of gene therapy vectors.• Perform down-stream process development like cell lysis, clarification, protein purification using AKTA pure and Pilot chromatography system, Tangential flow filtration• Assay development, Host cell protein and DNA analysis using ELISA, qPCR and fluorescence-based assays, endotoxin analysis, bioburden, and sterility testing.• Performed key role in bringing Isolere Bio’s first product in the market• Performed tech transfer of lab scale protein purification process to external CDMOs for large scale manufacturing• Cordination with other internal and external teams for application and process development• Train and advise junior scientists in their projects.
EDUCATION
Devi Ahilya Vishwavidyalaya
M.Sc., Biotechnology
IISER Bhopal
Doctor of Philosophy - PhD, Biology/Biological Sciences, General
Saurashtra University, Rajkot
B.Sc., Biotechnology
TATA high school Bilimora
High School Diploma, Biology/Biological Sciences, General
ABOUT SAURABH MEHTA
Cell and Developmental Biologist with more than 10 years of experience in following techniques:Cellular & Molecular Biology:• In-vivo and Ex-vivo RNAi screening • CRISPR, Gene knock-in, and knock-out studies • Transformation and Transfection, Stable cell line generation• Bacterial culture handling • Animal handling • DNA cloning including Gateway cloning, Gibson Assembly, Electrophoresis, PCR, qPCR/RT-PCR, Semi-quantitative PCR, Site-directed mutagenesis.• In-vitro Transcription, RNA isolation, and Reverse-Transcription cDNA synthesis Protein Biochemistry:• Expression of recombinant proteins in Bacterial and Insect cell hosts• Protein Purification by Affinity, Size-exclusion, Ion-Exchange chromatography using manual as well as AKTA-FPLC System• Protein quantitation using Lowry, Bradford, BCA, UV Spectrometry, and Qubit Assays• Characterization of proteins using Native and SDS-PAGE, Western Blotting, ELISA, Dot-blot assay, UV-VIS Spectroscopy• Enzyme isolation, purification and characterization, Steady-state kinetics analysis, mechanism-of-inhibition assay• Antibody generation, purification, and characterization• DNA-Protein Interaction analysis using non-radioactive EMSA and Chromatin-Immunoprecipitation Assay• Protein interactome analysis using Immunoprecipitation, and Proximity based Biotin-ligation Assay Microscopy:• Confocal microscopy using all major confocal systems, Leica, Zeiss, Olympus, Nikon, and Bio-Rad• High and Super-Resolution Microscopy using Zeiss Airy-Scan and Leica STED Microscopy systems• Confocal microscopic assays using Immunofluorescence, co-localization analysis, FRAP, FRET, Lambda-scanning, Fluorescence lifetime measurements, 3D-volumetric analysis • DNA and RNA Fluorescence In-situ Hybridization (FISH)• Image Processing and Analysis using ImageJ (Fiji), Zen, Imaris, and Volocity algorithms• Scanning Electron Microscopy sample processing and ImagingBioinformatics & Computational Skills:• DNA Plasmid design Snapgene, ApE, VectorNTI, and NEBuilder tools• DNA Sequence analysis using BLAST, Pairwise, and CLUSTAL W, T-Coffee• CRISPR design using various bioinformatics tools• Protein Structure Analysis using Phyre2, SMART, and Antigenicity prediction• Homology modeling using Phyre2 and SWISS-MODEL • Statistical analysis using GraphPad Prism and Microsoft Excel• Microsoft Office Suite; Adobe Photoshop, Illustrator, and Bio-render software• Reference management software EndNote and Mendeley• Literature survey using PubMed, Scopus, and Google Scholar• R programming language (working knowledge)
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