Radha Kapgate
MS in Molecular Pharmacology | Research Associate 2 at Seattle Children’s hospital | Drug and target validation | Translational Research
- Role
- Research Associate 2 at Seattle Children's
- Location
- Seattle, WA, US
- LinkedIn followers
- 500 followers
About Radha Kapgate
Results-driven researcher with a Master’s degree in Molecular Pharmacology and…
Experience
Research Associate 2
Aug 2024 — Present · Seattle, WA, US
PI: Dr. Jonathan Tang• Developed enzyme-based random mutagenesis to generate the library of nanobodies and sorted mutations using FACS, contributing to ongoing research for a proposed grant.• Successfully produced retroviral particles with 75% improved transduction efficiency in 293T cells; validated via fluorescence microscopy.• Analyzed sequencing data from 50+ samples using APE and BLAST to assess variability in nanobody sequences.• Ear-punched and optimized PCR-based protocols for the lab to genotype over 200 mice across >5 experimental groups.• Documented protocols and managed regulatory approvals to ensure compliance with institutional and federal standards; oversaw lab operations, including inventory management and safety protocols.
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