Petro Halkowycz

Research Senior Scientist, Biochemistry, Inflammation Drug Discovery @Takeda

Cambridge, MA, US

Represented the In Vitro Pharmacology group on multiple project core teams simultaneously as a decision maker on screening, SAR and mechanistic assays.• Experience with a variety of disease targets (GPCR, Integrins, Cytokines, Kinases, Proteases, Lipases, Carboxylases, etc.).• Developed and optimized biochemical assays using a broad variety of assay technologies including AlphaScreen, TR-FRET, FP, FI, Absorbance, Luminescence, and RLB.• Characterized proteins and compound binding using biophysical assays such as Thermal Shift assay (DSF).• Developed and conducted primary biochemical inhibition, activation and binding studies for hit identification and lead optimization.• Performed mechanistic studies for both slow binding reversible and covalent compounds using TR-FRET, FP and SPR technologies.• Developed and conducted fragment library screens where we identified and validated hits to initiate chemistry for multiple projects.• Developed and enabled assays for HTS screens and oversaw several externally conducted HTS campaigns where we identified and validated hits to initiate chemistry.• Developed and conducted selectivity, promiscuity and turbidity assays and screens.• Worked on late stage clinical projects performing mechanistic studies, selectivity and promiscuity studies, and reporting.• Oversaw development and execution of multiple biochemical assays with 10+ external partners.

University of Rochester

Bachelor's degree, Biology, General