Parinati Kharel
Senior Scientist I at Nitto BioPharma, Inc
- Role
- Senior Scientist i at Nitto Biopharma
- Location
- San Diego, CA, US
- LinkedIn followers
- 500 followers
About Parinati Kharel
Molecular biologist with decade of experience in academic, CRO and industry settings. Proficient in gene editing using CRISPR/Cas9 technology.Extensive experience in molecular and cellular techniques (cloning, gene synthesis, sanger sequencing, sequence analysis, RT-qPCR, Elisa, western blot, lentivirus production, cytochemistry, immunohistochemistry, reporter assays). Hands on experience in recombinant protein engineering, protein expression, protein purification (GE AKTA Pure/AVANT FPLC, Unicorn software) from bacterial and mammalian system, and protein characterization using biochemical and analytical techniques. Ability to independently design, plan, execute and interpret the experiments.
Experience
Senior Scientist i
May 2021 — Present · San Diego, CA, US
Support mRNA DDS (drug delivery system) platform development. Responsibilities include setting up molecular biology lab, designing new constructs, applying sequence engineering method to improve mRNA expression, optimizing in vitro transcription reaction to increase the yield and integrity of RNA, investigating the effect of different modifications on mRNA expression and stability and developing QC method to assess quality and integrity of mRNA
Education
The University of Texas at Arlington
Bachelor's degree
Southern Illinois University, Carbondale
Master of Science (M.Sc.)
Skills
- Qpcr
- Genotyping
- Rt-Pcr
- Tissue Culture
- Radiolabeling
- Itasser
- Rna Isolation
- Shrna
- Dna Extraction
- Gene Targeting
- Cmct
- Site-Directed Mutagenesis
- Limited Dntp
- Genetics
- Histology
- U Reaction
- Transfection
- Team Leadership
- Emsa
- Pcr Primer Design
- Jalview
- Bioinformatics
- Southern Blot
- Pcr
- Transgenic Mice
- Elisa
- Public Speaking
- Biotechnology
- Protein Purification
- Real-Time Pcr
- Immunostaining
- In Vivo Transcription/Translation
- Primer Design
- Northern Blotting
- Dna Sequencing
- Molecular Cloning
- Swiss Pdb Viewer
- Clustalw
- Western Blotting
- Gel Electrophoresis
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