Nitin Mandloi

Bioinformatics Scientist @Medgenome

Foster City, CA, US

Single-cell analysis: Expertise in single-cell gene expression, cell surface protein profiling, chromatin accessibility, immune profiling, and Visium spatial gene expression analysis.Long-read sequencing: Pacbio WGS analysis (Variant detection, assembly, epigenetics, Somatic variantsTumor/Normal samples), Kinnex full-length RNA, Kinnex single-cell RNA, PureTarget repeat expansion, target enrichment analysis.RNA-Seq: Differential expression analysis, pathway analysis, variant calling, gene fusion detection, HLA typing, and splicing event analysis.ChIP-Seq/Cut & Run: Chromatin immunoprecipitation and CUT & RUN sequencing for studying protein-DNA interactions.Bulk BCR/TCR clonotype profiling: Comprehensive immune repertoire analysis.Exome and whole-genome data analysis: Identification and annotation of SNPs/Indels, structural variants, copy number variants, somatic variants, annotations, report generation and visualization.Genome assembly and annotation.Small RNA sequencing data analysis.16S and Whole-genome metagenomicsGWAS association study: Expertise in data quality control and statistical analysis for genetic case-control and family-based association studies.Pipeline development, automation, containerization, and cloud computing.Development and automation of end-to-end bioinformatics pipelines, including RNA-seq, single-cell, spatial, methylation, exome/WGS, CUT & Tag/ChIP-seq, 16S, bacterial genome sequencing, small RNA sequencing, genome assembly and annotation.Implementation of various process automations, including de-multiplexing, data sharing via local FTP, AWS, Google Cloud, and Globus, data analysis, and interactive report generation and visualizations.

Manipal Life Science Centre,Manipal,(Karnataka)

M.Sc., Bioinformatics

Govt. Holkar Science College, Indore

B.Sc., Bioinformatics