Nitin Mandloi

Experience

1. Bioinformatics Scientist

   Medgenome

   Apr 2021 — Present · Foster City, CA, US

   Single-cell analysis: Expertise in single-cell gene expression, cell surface protein profiling, chromatin accessibility, immune profiling, and Visium spatial gene expression analysis.Long-read sequencing: Pacbio WGS analysis (Variant detection, assembly, epigenetics, Somatic variantsTumor/Normal samples), Kinnex full-length RNA, Kinnex single-cell RNA, PureTarget repeat expansion, target enrichment analysis.RNA-Seq: Differential expression analysis, pathway analysis, variant calling, gene fusion detection, HLA typing, and splicing event analysis.ChIP-Seq/Cut & Run: Chromatin immunoprecipitation and CUT & RUN sequencing for studying protein-DNA interactions.Bulk BCR/TCR clonotype profiling: Comprehensive immune repertoire analysis.Exome and whole-genome data analysis: Identification and annotation of SNPs/Indels, structural variants, copy number variants, somatic variants, annotations, report generation and visualization.Genome assembly and annotation.Small RNA sequencing data analysis.16S and Whole-genome metagenomicsGWAS association study: Expertise in data quality control and statistical analysis for genetic case-control and family-based association studies.Pipeline development, automation, containerization, and cloud computing.Development and automation of end-to-end bioinformatics pipelines, including RNA-seq, single-cell, spatial, methylation, exome/WGS, CUT & Tag/ChIP-seq, 16S, bacterial genome sequencing, small RNA sequencing, genome assembly and annotation.Implementation of various process automations, including de-multiplexing, data sharing via local FTP, AWS, Google Cloud, and Globus, data analysis, and interactive report generation and visualizations.

Education

• Manipal Life Science Centre,Manipal,(Karnataka)

  M.Sc., Bioinformatics

  2010 — 2012

• Govt. Holkar Science College, Indore

  B.Sc., Bioinformatics

  2007 — 2010

Skills

• Agilent Acgh
• Illumina Gene Expression
• Illumina Dna Methylation
• Sequence Analysis
• Ngs Data Analysis
• Computational Biology
• Genomics
• Genome-Wide Association Analysis
• Bioinformatics
• Illumina Snp Genotyping