Nikhil Vad

WORK HISTORY

EDUCATION

SKILLS

ABOUT NIKHIL VAD

Immunotherapy,#Celltherapy,#Immunooncology #antibodytherapeuticsTechnical skillsHTS, compound profiling, target identification/validation, lead identification/optimization, proliferation assays, reporter assays, signal pathways, mechanism of action studies, drug resistance pathways, cancer stem cell isolation, assays with primary & stable cell lines, kinase assays, cell- and enzyme-based biochemical assays, ELISA, IHC/IF, RNAi, qPCR, microRNA isolation and analyses, MACS cell separation, bead-based immunoassays, rodent dosing and necropsy, toxicology, in vivo efficacy studies.Highlights• Using HTS, explored activity profiles of chemical libraries targeted against key proteins in the autophagy pathway for hit identification and optimization.• Harnessed synthetic lethal screens to identify target-specific cytotoxic agents against brain tumors.• Executed cell-based phenotype screens (image-based, reporter-based) to facilitate rapid identification of candidates likely to exhibit combinatorial activity with lead compound.• Determined whether the presence of mutant PIK3CA or KRAS influenced the growth inhibitory activity of MEK or PI3K inhibitors. Assessed cell proliferation, tumor growth, with and without drug treatment in 2D and 3D cultures in vitro, and as xenografts in immunocompromised mice in vivo.• Used FACS analysis for phenotype assessment of compound-treated multiple myeloma cells including cells isolated from BM and PBMCs of patients.• Used HTS, in vitro (cell- and enzyme-based) and ex vivo (isolated rat hepatocytes, induced rat/mouse liver microsomes) biochemical screens on a chemical library for compound profiling, lead identification/optimization. Mechanism of action studies accomplished using genetic or pharmacological manipulation of the biological target to demonstrate target dependency/proof of biology. In vitro observations confirmed using pharmacodynamic endpoints in mouse models.