Nidhi Tiwari

WORK HISTORY

EDUCATION

ABOUT NIDHI TIWARI

As a meticulous biotechnologist with a proven track record of producing significant outcomes, I specialize in molecular biology, cloning, and polymer science. My passion for biotechnology drives me to employ critical thinking in experimental design and data analysis to generate new insights. I am well-versed in standard regulations, adept at performing lab operations dexterously, and effective in collaborating across multi-disciplinary teams.With a robust academic background and extensive research experience, I have honed my skills in various biotechnological domains. My current research at the German Center for Diabetes Research focuses on the impact of SARS-CoV-2 non-structural protein 1 (Nsp1) on proteins of β-cells, potentially elucidating mechanisms behind insulin secretion deficiencies during infection. Additionally, my work at the Center for Regenerative Therapies investigates the effects of TM6SF2 deficiency in HepG2 cells, contributing to our understanding of non-alcoholic fatty liver disease (NAFLD).Research and Technical SkillsCell Biology-Stem cell culture, cell line maintenance, cell splitting, cell counting, C. elegans handlingBiochemistry-Western blotting, enzymatic assay, reporter gene assay, BCA assay, ion-exchange chromatographyMolecular Biology-Cloning, PCR, RT/qPCR, transfection, mini prep, RNA isolation, SDS-PAGE, Gibson AssemblyPolymer Science-Spin coating, laser grafting, IR spectroscopy, colorimetric assay, spectrophotometric assay°Research Highlights Nsp1 Impact on β-Cells-Utilized cloning, cell culture, transfection, RNA extraction, RT-PCR, Q-PCR, western blot, and immunostaining to study SARS-CoV-2 Nsp1’s suppression of host protein synthesis.Surface Modification of Thermoplastics- Conducted a parametric study on laser-assisted surface modification grafting of PEI on PETG, using UV-VIS spectroscopy to identify optimal laser additives.I believe in the importance of continuous learning and staying abreast of the latest advancements in biotechnology. As a collaborative team player with a keen eye for detail and a commitment to scientific integrity, I strive to contribute to impactful research and innovation in the field.I am always open to connecting with fellow professionals and exploring new opportunities for collaboration. Feel free to reach out to discuss potential projects or share insights on the latest advancements in biotechnology.