Nicholas Ruiz
Cell & Molecular Biology Major | UC San Diego
- Role
- Scientific Intern, Rna Process Development at Arcturus Therapeutics
- Location
- San Diego, CA, US
- LinkedIn followers
- 500 followers
About Nicholas Ruiz
I’m a premedical student at UC San Diego exploring the field of medicine through…
Experience
Scientific Intern, Rna Process Development
Nov 2024 — Present · San Diego, CA, US
Supported development and scale-up of mRNA synthesis and purification processes-Conducted in vitro transcription (IVT) reactions, experimenting with different factors and conditions to optimize RNA yield, purity, and quality-Performed chromatography techniques using AKTA and HPLC, including method development and optimization for RNA and protein purification-Utilized analytical instruments such as HPLC, UV-Vis spectrophotometer, and capillary electrophoresis (CE) to assess RNA integrity and purity-Quantified RNA concentration and purity using RiboGreen fluorescent dye assay-Conducted RNA labeling and ligation for experimental applications-Ran SDS-PAGE and native PAGE for protein analysis and process characterization-Maintained and handled mammalian cell cultures, including seeding, passaging, and preparation for downstream assays-Performed general analytical assays using spectrophotometry, plate readers, and gel electrophoresis-Collaborated across research and production teams to support multiple projects and deliver high-quality results.
Education
University High School
High School Diploma
UC San Diego
Bachelor of Science - BS, Cell/Cellular and Molecular Biology
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