Nattakan Sukomon

Experience

1. Scientist

   Evive Biotech

   Dec 2022 — Present · Hayward, CA, US

   Protein Engineering, Expression and Characterization• Designed antigen constructs for immunization and antibody discovery campaigns• Expressed antigens and antibodies in monoclonal, VHH and bispecific formats; purified proteins using affinity purification, size exclusion chromatography and ion exchange purification methods• Established antibody humanization protocol using Molecular Operating Environment (MOE); • Developed in silico antibody analysis protocols for developability assessment and designed mutations to eliminate developability issues• Set up automated protein purification and normalization via EpMotion• Characterized protein kinetics, affinity and competition using BLI and SPR• Assessed antigen and antibody properties using analytical size exclusion chromatography, differential scanning fluorimetry (DFS), capillary isoelectric focusing (cIEF) electrophoresis and capillary electrophoresis (CE-SDS)Molecular Biology Lead• Established molecular biology lab; developed protocols for DNA cloning and mutagenesis • Generated intermediate toolboxes to facilitate antibody engineering• Maintained molecular biology inventories and data • Supervised and trained Research Assistant on molecular biology techniqueProject lead• Led cytokine-based fusion protein project and collaborated with team members across various functions• Designed protein constructs; established cell-based assays using PBMC and isolated immune cells to determine efficacy and drug properties of designed proteins• Cooperated CROs for designing in vivo study protocols and followed up with experimental progress

Education

• Cornell University

  Doctor of Philosophy (Ph.D.)

  2010 — 2016

• King Mongkut's Institute of Technology Ladkrabang

  Master’s Degree

  2006 — 2008