Natasha Sieczkiewicz
Research Assistant @University of Rochester Medicine
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WORK HISTORY
Research Assistant @University of Rochester Medicine
Rochester, NY, US
I am currently working as an Undergraduate Research Assistant in the Kielkopf Lab at the URMC. I am starting a project where I will investigate the role of reactive oxygen species (ROS) in regulating PPM1D phosphatase. PPM1D mutations or overexpression suppress DNA damage responses and p53-mediated apoptosis, promoting chemoresistance. It is hypothesized that ROS-sensing cysteine residues uniquely positioned in PPM1D modulate its activity through redox-dependent structural changes. My experiment will first characterize how oxidation alters PPM1D’s enzymatic function and cellular stability using biochemical assays, cysteine-mimetic mutants, and redox proteomics. After this, I will then screen covalent small-molecule libraries targeting these cysteines, which could be used to develop selective PPM1D inhibitors. These hits will also be validated in orthogonal cell-based models measuring p53 activation, substrate dephosphorylation, and chemosensitization.
EDUCATION
University of Rochester
Bachelor's degree, Molecular Genetics
Lewisburg Area High School
High School Diploma
Vrije Universiteit Amsterdam (VU Amsterdam)
Molecular Biology
ABOUT NATASHA SIECZKIEWICZ
My name is Natasha Sieczkiewicz, and I am student at the University of Rochester currently pursuing a BS in Molecular Genetics and a minor in Anthropology. I am passionate about understanding the complexities behind molecular mechanisms and how this understanding can be applied to studies regarding genetic diseases and conditions. After graduating from the University of Rochester, I aim to work in a research laboratory and gain experience before ultimately pursing a PhD in genetics, and eventually work for a company where my research could be used to enhance the lives of others.I currently work as an undergraduate researcher in the Kielkopf lab at the URMC. I am starting a project where I will investigate the role of reactive oxygen species (ROS) in regulating PPM1D phosphatase. PPM1D mutations or overexpression suppress DNA damage responses and p53-mediated apoptosis, promoting chemoresistance. It is hypothesized that ROS-sensing cysteine residues uniquely positioned in PPM1D modulate its activity through redox-dependent structural changes. Right now, I am first characterizing how oxidation alters PPM1D’s enzymatic function and cellular stability using biochemical assays, cysteine mutants, and redox proteomics. Beyond academics, I am drawn to other learning experiences that strengthen my academic goals. I have worked as an undergraduate TA for a genetics course and another introductory biology course focusing on ecology and evolution. Additionally, my past employment experiences have equipped me with the skills I need to succeed in new positions and the mindset to strive to understand the world around me.
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