Natalie H.

About Natalie H.

I have over 10 years of experience working with E. coli cultivation and genetic engineering and 4 years of experience in the biotech industry.Recently, I worked at an environmentally focused, small biotech startup where I regularly practiced and defined my critical thinking skills, planning and executing efficient experiments, and defined my understanding of how I communicate and work with a team. I have the opportunity to work with every scientific aspect of our company from molecular biology to protein chemistry. Additionally, I gained necessary skills in laboratory management and set up.Previously, I worked in a medium-sized biotech company where I regularly performed high-throughput microtiter plate fermentation of E. coli. I cross-trained with teams running Ambr 250mL bioreactors, 20L NLF fermentation tanks, and sequence development. I also developed the media library in the new laboratory information management system.Competencies: Lab management; High-throughput microtiter plate fermentation; Geneious; JMP; E. coli cultivation; Aseptic culturing; Transient expression assays; DNA extraction- microbes, and fungi; Protein and RNA extraction- plants; Indoor growth chambers; Media preparation; Rhizosphere and endosphere preparations; Cryopreservation; PCR; Gel electrophoresis; Northern blot analysis; Western blot analysis; Plasmid cloning; Agrobacterium transformation; Fungal isolations; Microsoft Office; Adobe Illustrator; Adobe Photoshop; Image J

Experience

1. Associate Scientist

   Novel Bio

   Oct 2024 — Present · Vancouver, WA, US

   Operations:High-Quality (cGMP-Aligned Manufacturing) Scale- Head of Quality Control- Main producer of pDNA using only single-use equipment- Ensures all materials throughout the production of pDNA are BSE/TSE compliant- Lead the initial high-quality pDNA production workflow from materials management, to colony screening, scale-up, to purification, QC, to shipment (SEP2025 - DEC2025)R&D Scale- Cloning, Cultivation, Large-Scale DNA purification, and QC of E. coli and the NBx Platform™- CyClone™ product production and QC- Authors majority of Certificate of Analyses- Coordinates CyClone™ and pDNA shipments (NOV2024 - DEC2025)Research and Development- Optimized Bacterial Endotoxin Testing (BET) in pDNA with cartridges by Charles River. Successfully switched from LAL to using only animal-free Trillium™ rCR- Developed Essential QC Assays including Residual RNA, Residual Genomic DNA, Residual Protein, Visual Appearance, Plate-Based Endotoxin detection- Lead Researcher of a new pDNA synthesis platform (JAN - AUG2025)- Associate Strain Engineer on the NBx Platform™ (DEC2024 - JUN2025)Instruments: Endosafe® nexgen-PTS™; Infors HT Multitron Pro, PetriSwiss, QuantStudio Absolute Q Digital PCR SystemNew Techniques: Bacterial Endotoxin Testing, CRISPR-Cas9, Natural Transformations of Vibrio natriegens, Plasmid Curing, DNA precipitation, Digital PCR, Residual RNA detection, Machery-Nagle Chromabond DNA purification, Residual Protein detection (BCA)

Education

• Missouri University of Science and Technology

  Bachelor’s Degree, Biology/Biological Sciences, Minor in Chemistry and Biomedical Engineering

  2013 — 2017

• Romeoville High School

  General Studies, Biology, General

  2009 — 2013

• University of Nebraska-Lincoln

  Master of Science - MS, Plant Pathology/Phytopathology

  2018 — 2020

Skills

• Science
• Biochemistry
• Research
• Genetics
• Molecular Biology
• Corn Development and Staging
• Badminton
• Sustainable Agriculture
• Cell Biology
• Cell Culture
• Data Analysis
• Chemistry
• Waiting
• Tennis
• Statistics
• Cover Crops
• Biodiversity
• Radioactivity
• Anatomy
• Microsoft Word
• Piano
• Teamwork
• Soybean Development and Staging
• Biotechnology
• Microsoft Powerpoint
• Secretarial Skills
• Microsoft Office
• Microsoft Excel
• Powerpoint
• Biological Sciences
• Biology