Nadine Keller

About Nadine Keller

Highly motivated and detail-oriented professional (PhD) with strong work ethic and sound knowledge/expertise in diverse life science areas, both hands-on in a wet lab and as a project manager/team leader• Strong background in biochemistry and molecular biology - Recombinant nucleic acid technology (DNA/RNA isolation, cloning, site-directed mutagenesis, library construction), Sanger sequencing, protein expression (from bacteria or mammalian cells, soluble, as inclusion bodies, isotope labeling), protein purification (affinity/size exclusion/ion exchange chromatography; Äkta/FPLC systems), antibody development, phage display, yeast two-hybrid screening, ribosome display, SDS-PAGE, Western blot, Southern blot, ELISA • Expertise in cell biology- Generation and maintenance of human and mammalian cell lines (transient and stable transfections, viral infections, cryo preservation), development and execution of functional cell-based assays including survival and cell death assays for drug screening, RT-PCR, qPCR, FACS/flow cytometry, microscopy (confocal, fluorescence, immunofluorescence)• Experienced in production process development and validation in GLP and GMP environment• Proficient in the development of SOPs• Exemplary analytical and problem solving skills • Efficient organization, planning, optimization and multitasking skills to ensure on time delivery• Excellent interpersonal, oral and written communication and presentation skills • Adept at building and maintaining excellent relationships with coworkers and collaborators • Effective in working autonomously or collaboratively in team-oriented, cross-functional and fast-paced environments• Successfully trained and supervised groups of up to 5 people, fostering their motivation and team commitment • Experience in lab management: database maintenance, negotiations with vendors, reagent ordering, equipment supervision, ensuring lab safely• Excellent computer literacy

Experience

1. Sr Manager, Enzymes Process Development

   Singular Genomics

   Oct 2023 — Present · San Diego, CA, US

Education

• Technical University of Berlin

  Diploma, Chemistry

  1998 — 2004

• University of Oklahoma

  Master's degree, Biochemistry

  2000 — 2002

• University of Zurich

  Doctor of Philosophy (PhD), Biochemistry

  2004 — 2009

Skills

• Flow Cytometry
• Protein Expression
• Western Blotting
• Team Leadership
• Site-Directed Mutagenesis
• Nmr Spectroscopy
• Enzyme Kinetics
• Protein Purification
• Biochemistry
• Molecular Cloning
• Fplc
• Project Management
• Analytical Ultracentrifugation
• Structural Biology
• Cell Biology
• Qpcr
• Laboratory Managment
• Confocal Microscopy
• Illustrator
• Molecular Biology
• Fluorescence Microscopy
• Elisa
• Akta
• Microsoft Office
• Photoshop
• Lentivirus
• Recombinant Dna Technology
• Cell Migration
• Cell Culture
• X-Ray Crystallography
• Southern Blot
• Tissue Culture