Mohammad Kashem
Research Scientist @Government Of Canada
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WORK HISTORY
Research Scientist @Government Of Canada
Research and development: Conducting research in Foot-and-Mouth Disease (FMD), particularly in diagnostic and vaccine development.
EDUCATION
Chattogram Veterinary and Animal Sciences University (CVASU)
Doctor of Veterinary Medicine, Veterinary Medicine
Chattogram Veterinary and Animal Sciences University (CVASU)
Master's degree, Microbiology
University of Manitoba
Doctor of Philosophy - PhD, Medical Microbiology
ABOUT MOHAMMAD KASHEM
Specialties:More than 14 years of experience in medical and veterinary microbiology, immunology, molecular biology, and virology, including HIV host genetics and FMD. Experienced in working in containment level II and laboratories.# Technical expertise:1. In vitro studies: cell culture, cell transfection, lentivirus production, lentiviral transduction, cloning, bacterial transformation, competent cell preparation, plasmid purification, CRISPR/Cas9 gene editing, and electroporation.2. In vivo studies: mouse models.3. Immunology: cell signaling pathways, B-cell and T-cell biology, and flow cytometry applications.4. Molecular biology: DNA/RNA isolation and purification, PCR primer design, conventional PCR, RT-PCR, real-time qPCR, gel electrophoresis, Nanopore sequencing, Sanger sequencing, 454 sequencing, genotyping, and sequence data analysis.5. Protein biology: protein purification, quantification, and detection, multiplex cytokine/chemokine assays, ELISA, Western blot analysis, including fluorescent Western blot, immunohistochemistry staining, and immunofluorescence staining.6. Microscopy: confocal and fluorescence microscopy.7. High-performance liquid chromatography (HPLC): SEC-HPLC.8. Viral propagation and diagnostic tests: virus cultivation in fertile eggs, virus neutralization test (VNT), agar-gel immunodiffusion assay, and hemagglutination inhibition assay.9. Data analysis software: GraphPad Prism, RStudio, FlowJo, Clustal Omega, FinchTV, Benchling, ImageStudio, ImageJ, Zeiss Zen Lite.# Experienced in teaching university undergraduate and graduate students.# Experienced in mentoring and supervising graduate students (thesis-based), conference participants, and academic staff.# Experienced in the peer-review process (journals and research grants).# A highly motivated, self-directed person with experience working in a team.# Strong critical thinker with the ability to use creativity to solve problems.# Excellent in oral communication, networking, reading, and writing (including scientific writing).
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