Mingrui A.
Director at Firefly Biologics
- Role
- Director at Firefly Bio
- Location
- South San Francisco, CA, US
- LinkedIn followers
- 500 followers
About Mingrui A.
Mass spectrometry: intact mass measurement of proteins; peptide mapping; PTMs analysis, such as oxidation, methylation, acetylation, phosphorylation, ubiquitination, glycosylation and disulfide bonds; quantitative proteomics (and phosphoproteomics) analysis using label free, SILAC or isobaric tag (TMT, iTRAQ) labeling strategies; targeted protein quantification by PRM; de novo sequencing of antibody; epitope mapping by HDX. • U/HPLC: protein purity measurement by SEC; hydrophobicity evaluation by HIC; fractionation using PR and IEX; titer measurement by Protein A/G chromatography. • Electrophoresis: cIEF using iCE3, Maurice and PA800 Plus; reduced and non-reduced CE-SDS by PA800 Plus and LabChip; SDS-PAGE. • Purification: small-scale antibody purification by gravity columns or HPLC-ProA • Other analytical skills: melting temperature (Tm) measurement by DSC and DSF; residual DNA determination using PCR, protein-protein interaction using BLI Gator and ELISA; Protein homogeneity measurement by multi-angle light scattering (MALS) and Dynamic Light Scattering (DLS)• Research and writing: 48 peer-reviewed publications & hl=en) and 15 conference proceedings
Experience
Director
Sep 2023 — Present · South San Francisco, CA, US
Education
Peking University
Doctor of Philosophy - PhD, Biochemistry and Molecular Biology
2007 — 2013
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